斑马鱼hoxa1a基因调控颅面骨骼发育的功能研究*

武秀知,王宏杰,祖尧

Functional Study of hoxa1a Regulating Craniofacial Bone Development in Zebrafish

Xiu-zhi WU,Hong-jie WANG,Yao ZU

图1 利用CRISPR/Cas9系统编辑斑马鱼hoxa1a基因

Fig.1 Using CRISPR/Cas9 system to edit zebrafish hoxa1a gene (a) CRISPR/Cas9 knockout pattern (b) Knockout efficiency of F0 was 70% on average (c) Screening for F1 zebrafish with inherited mutation using T7E1 (d) F1 heterozygous sequence alignment, screening +8 bp heterozygous and -7 bp heterozygous