Clinical Significance of Absolute Count of Peripheral Blood Immune Cells in Children with Immune Thrombocytopenia

doi:10.13523/j.cb.2310053

China Biotechnology

2023, Vol. 43

Issue (12): 128-133 DOI: 10.13523/j.cb.2310053

Clinical Significance of Absolute Count of Peripheral Blood Immune Cells in Children with Immune Thrombocytopenia

YANG Zhi-hong^1,^*(

),ZHANG Ya-qi²,BAI Yong-ze¹,SHI Zhi-yun¹,PAN Lin¹,CHEN Ping¹

1 Center of Medical Laboratory, The General Hospital of Ningxia Medical University, Yinchuan 750004, China
2 The First Clinical Medical School of Ningxia Medical University, Yinchuan 750004, China

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Abstract

Objective: To investigate the changes and clinical significance of the absolute count of peripheral blood immune cells in children with immune thrombocytopenia (ITP). Methods: A total of 41 newly diagnosed ITP children admitted to the Department of Pediatrics, General Hospital of Ningxia Medical University from January 2022 to August 2023 were selected as the case group, while 30 healthy children were selected as the control group. Flow cytometry was used to detect and compare the absolute count and percentage levels of CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺T cells, CD3^-CD19⁺B cells and CD3^-CD16⁺CD56⁺NK cells, as well as the CD4⁺/CD8⁺ratio in peripheral blood between the two groups. Results: The absolute count levels of CD3⁺ and CD3⁺CD4⁺T cells, as well as the absolute count and percentage levels of CD3^-CD19⁺B cells in the case group were significantly increased compared to the control group (P<0.05). The percentage of CD3^-CD16⁺CD56⁺NK cells significantly decreased (P<0.05). There was no statistically significant difference between two groups in the absolute count of CD8⁺T cells,the percentage of CD3⁺,CD4⁺,CD8⁺T cells, the ratio of CD4⁺/CD8⁺ and the absolute count of CD3^-CD16⁺CD56⁺NK cells (P>0.05). Conclusions: There is a disorder in the immune cells of peripheral blood in children with ITP. The absolute count level can objectively and truly reflect the changes of immune cells in children with ITP. Judging abnormal changes in immune cell levels should be based on the absolute count level to analyze the percentage level changes, so as to provide better assistance for clinical disease diagnosis and treatment.

Key words： Immune thrombocytopenia Lymphocyte subpopulations Absolute counting Immune cells

Received: 16 October 2023 Published: 16 January 2024

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	Zhi-hong YANG
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	Yong-ze BAI
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	Lin PAN
	Ping CHEN

Cite this article:

Zhi-hong YANG, Ya-qi ZHANG, Yong-ze BAI, Zhi-yun SHI, Lin PAN, Ping CHEN. Clinical Significance of Absolute Count of Peripheral Blood Immune Cells in Children with Immune Thrombocytopenia. China Biotechnology, 2023, 43(12): 128-133.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2310053 OR https://manu60.magtech.com.cn/biotech/Y2023/V43/I12/128

Table 1 The percentage and absolute count levels of peripheral blood CD3⁺T, CD4⁺T, CD8⁺T cells in the case group and the control group(

x -

±s)

Table 2 Percentage and absolute count levels of CD3^-CD19⁺B cells in peripheral blood of patients in the case group and control group(

x -

±s)

Table 3 Percentage and absolute count levels of CD3^-CD16⁺CD56⁺NK cells in peripheral blood of patients in the case group and control group(

x -

±s)


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