The CiNAC1 from <i>Caragana intermedia</i> Promotes Transgenic Arabidopsis Leaf Senescence

doi:10.13523/j.cb.20180404

China Biotechnology

2018, Vol. 38

Issue (4): 24-29 DOI: 10.13523/j.cb.20180404

The CiNAC1 from Caragana intermedia Promotes Transgenic Arabidopsis Leaf Senescence

Wen-ran YUE,Jun-yan YUE,Xiu-juan ZHANG,Qi YANG,Xiao-dong HAN,Rui-gang WANG,Guo-jing LI(

)

College of Life Sciences, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, Hohhot 010018, China

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Abstract

NAC transcription factors are one of the largest plant-specific transcription factor families, and play important roles in plant growth and development, such as biotic and abiotic stress response, hormone signaling pathway, plant secondary growth, cell division and plant senescence. The CiNAC1 transgenic Arabidopsis thaliana homozygous lines was compared with wild type for further functional analyzes. The results showed that CiNAC1 overexpression lines promoted leaf senescence after ethylene treatment,furthermore, the chlorophyll contents of leaves were remarkably lower in transgenic lines than in wild-type, and ion leakage increased in transgenic lines. Quantitative real time PCR analysis showed that the expression level of chlorophyll catabolic genes, SGR1, SGR2 and PPH as well as senescence related genes, such as SAG13, SAG29, ORE1, SINA1,VNI2 and EIN3, a key transcription factor in ethylene signaling, was significantly higher in transgenic lines than in wild-type under ethylene treayment. Taken together, these results indicate that CiNAC1 play a role in ethylene induced leaf senescence in A. thaliana.

Key words： NAC transcription factors CiNAC1 Ethylene Leaf senescence

Received: 26 October 2017 Published: 08 May 2018

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	Wen-ran YUE
	Jun-yan YUE
	Xiu-juan ZHANG
	Qi YANG
	Xiao-dong HAN
	Rui-gang WANG
	Guo-jing LI

Cite this article:

Wen-ran YUE,Jun-yan YUE,Xiu-juan ZHANG,Qi YANG,Xiao-dong HAN,Rui-gang WANG,Guo-jing LI. The CiNAC1 from Caragana intermedia Promotes Transgenic Arabidopsis Leaf Senescence. China Biotechnology, 2018, 38(4): 24-29.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180404 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I4/24

Fig.1 A working model of the EIN3-ORE1-CCGs in regulation of ethylene-mediated chl degradation

Fig.2 The expression of CiNAC1 under ethylene treatment by qRT-PCR

Fig.3 The senescence phenotypes of WT (wild type) and transgenic CiNAC1 Arabidopsis plant

Fig.4 Ethylene induced leaf senescence of 4-weeks-old WT and transgenic CiNAC1 Arabidopsis plants

Fig.5 Chlorophyll contents and membrane ion leakage of WT and transgenic CiNAC1 Arabidopsis plants

Fig.6 Expression of leaf senescence related genes and chlorophyll catabolic genes in transgenic CiNAC1 Arabidopsis plants


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