|
|
|
| Construction of a Neotype Controllability Magnetic Targeting Restructuring Truncated Tissue Factor |
| CHEN Xiao-li1, ZOU Ming-yuan1, XIE Xin3, WANG Sheng-yu1, WU Ting1, SU Jin-hua2 |
1. Cancer Research Center, Medical College, Xiamen University, Xiamen 361005, China;
2. Laboratory Animal Center, Xiamen University, Xiamen 361005, China;
3. Department of Oncology, Chenggong Hospital Affiliated to Xiamen University, No. 174 Hospital of People's Liberation Army, Xiamen 361000, China |
|
|
|
Abstract Objective:To establish a neotype controllability magnetic targeting restructuring truncated tissue factor s for establish animal models of thrombosis. Methods:Magnetic nanoparticles (MNPs) were prepared by chemical coprecipitation, and employed the superconducting quantum interference device to characterized the MNPs magnetization. The truncated tissue factor (tTF) were expressed efficiently in E.coli and purification by gene engineering technology. MNPs conjugated the tTF by glutaraldehyde to compose MNPs-tTF. In vitro analysis the effects of magnetic targeting and blood coagulation of MNPs-tTF. Result:The magnetic targeting restructuring truncated tissue factor composites were build successfully. They are reserve efficient coagulation effect and magnetic targeted ability in vitro. Conclusion:We have successfully prepared magnetic targeting thrombin system MNPs-tTF have been successfully prepared, which maybe provide a basis for developing the establish animal models of thrombosis.
|
|
Received: 17 April 2017
Published: 25 September 2017
|
|
|
|
|
[1] Cooley B C. Murine arterial thrombus induction mechanism influences subsequent thrombodynamics. Thrombosis Research, 2015,135(5):939-943.
[2] Guven M, Aras A B, Akman T, et al. Neuroprotective effect of p-coumaric acid in rat model of embolic cerebral ischemia. Iranian Journal of Basic Medical Science, 2015,18(4):356-363.
[3] Lei Z, Pang X, Li N, et al. A novel two-step modifying process for preparation of chitosan-coated Fe3O4/SiO2 microspheres. Journal of Materials Processing Tech, 2009. 209(7):3218-3225.
[4] Ruf W, Rehemtulla A, Morrissey J H,et al. Phospholipid-independent and-dependent interactions required for tissue factor receptor and cofactor function. Journal of Biological Chemistry, 1991,266(4):2158-2166.
[5] Stone M J, Ruf W, Miles D J, et al. Recombinant soluble human tissue factor secreted by Saccharomyces cerevisiae and refolded from Escherichia coli inclusion bodies:glycosylation of mutants, activity and physical characterization. Biochem J,1995,310(Pt 2):605-614.
[6] Zhou F, Chen L, An Q, et al. Novel hydrogel material as a potential embolic agent in embolization treatments. Scientific Reports, 2016,6:32145.
[7] Berenstein A. Treatment of experimental aneurysms with a new liquid embolic agent and a retrievable stent:proof of concept and feasibility study. Journal of Neurointerventional Surgery, 2016,8(9):934.
[8] Leyon J J, Chavda S, Thomas A, et al. Preliminary experience with the liquid embolic material agent PHIL (precipitating hydrophobic injectable liquid) in treating cranial and spinal dural arteriovenous fistulas:technical note. Journal of Neurointerventional Surgery, 2015,8(6):596-602.
[9] Brassel F, Meila D. Evolution of embolic agents in interventional neuroradiology. Clinical Neuroradiology, 2015,25(2):333.
[10] An Y, Jiang G, Qi Y, et al. Synthesis of nano-plugging agent based on AM/AMPS/NVP terpolymer. Journal of Petroleum Science & Engineering, 2015,135:505-514.
[11] Choi S Y, Kwak B K, Shim H J,et al. MRI traceability of superparamagnetic iron oxide nanoparticle-embedded chitosan microspheres as an embolic material in rabbit uterus. Diagnostic & Interventional Radiology, 2015,21(1):47.
[12] 张永永. pH响应聚氨基酸的合成、性能及在肿瘤栓塞中的应用. 兰州:西北师范大学医学院,2016. Zhang Y Y.Synthesis Properties and Application in Tumor Embolism of pH-responsive Poly(amino acid).Lanzhou:Medical College,Northwest Normal University,2016.
[13] 谭衍, 边远, 汤树洪,等. 液体栓塞剂在颅内动脉瘤栓塞中的应用. 广西医科大学学报, 2015,32(2):299-301. Tan Y, Bian Y,Tang S H,et al. Application of liquid embolization in intracranial aneurysm embolization. Journal of Guangxi Medical University, 2015, 32(2):299-301.
[14] 李成, 施昌盛, 虞希祥,等. Embosphere微球靶血管栓塞治疗中晚期肺癌大咯血的疗效. 中华介入放射学电子杂志, 2016,4(3):155-157. Li C, Shi C H, Yu X X, et al. Evaluation on the efifcacy of the target-vessel embolization with Embosphere microspheres for the treatment of massive haemoptysis in advanced lung cancer. Chinese Journal of Interventional Radiology, 2016, 4(3):155-157.
[15] 姚群立, 陆建东, 徐向荣, 等. 不同栓塞剂栓塞子宫动脉治疗子宫腺肌症的中远期随访分析. 介入放射学杂志, 2015(3):248-252. Yao Q L, Lu J D, Xu X R, et al. Uterine artery embolization for the treatment of uterine adenomyosis:comparison study of different embolic agents. Journal of Interventional Radiology, 2015(3):248-252. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
