Targeting TRIM5α Gene of CD4<sup>+</sup> T cells of <em>Macaca mulatta</em> Affect Their Ability on HIV Infection

doi:10.13523/j.cb.20170203

China Biotechnology

2017, Vol. 37

Issue (2): 15-19 DOI: 10.13523/j.cb.20170203

Targeting TRIM5α Gene of CD4⁺ T cells of Macaca mulatta Affect Their Ability on HIV Infection

WANG Xiao-li^1,2, YU Qing², YUAN Ya-hong^1,2, TENG Zhi-ping³, LI Dong-sheng², ZENG Yi¹

1. College of Life Science, Beijing University of Technology, Beijing 100124, China;
2. Life Science Institute, Affiliated Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China;
3. Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China

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Abstract

Objective: To study the effect of tripartite motif 5 alpha (TRIM5α) on the HIV-1 infection of CD4⁺ T cells of Macaca mulatta. Methods: Separation CD4⁺ T cells from peripheral blood of Macaca mulatta by magnetic cell sorting and the rate of CD4⁺ T cells was detected by flow cytometry. The transcription activator-like effector nuclease (TALEN) plasmids which targeted TRIM5α gene were constructed and transfected into CD4⁺ T cells by electroporation. The cells which transfected into TALEN plasmids were separated by flow cytometry. The genome of the targeted cells was extracted and analyzed by T7E1 enzyme digestion. The HIV-1 virus were used to infect the CD4⁺ T cells which were targeted TRIM5α and the ability of virus infection was detected by ELISA. Results: The CD4⁺ T cells were successfully separated from the peripheral blood of Macaca mulatta and the positive rate was 99.5%. The TRIM5α TALEN plasmids could enter the CD4⁺ T cells by electroporation, the infection efficiency was 24.8% and the target efficiency was 40%. The result of ELISA showed that the HIV-1 virus could infect the CD4⁺ T cells of Macaca mulatta which were targeted TRIM5α. Conclusion: Targeting TRIM5α gene of CD4⁺ T cells of Macaca mulatta can increase its ability to infect HIV-1 virus. This will lay the foundation for the further establish the Macaca mulatta model for HIV-1 study.

Key words： CD4⁺ T HIV-1 infection Tripartite motif 5 alpha Macaca mulatta

Received: 12 August 2016 Published: 25 February 2017

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Cite this article:

WANG Xiao-li, YU Qing, YUAN Ya-hong, TENG Zhi-ping, LI Dong-sheng, ZENG Yi. Targeting TRIM5α Gene of CD4⁺ T cells of Macaca mulatta Affect Their Ability on HIV Infection. China Biotechnology, 2017, 37(2): 15-19.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170203 OR https://manu60.magtech.com.cn/biotech/Y2017/V37/I2/15

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