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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (5): 68-73    DOI: 10.13523/j.cb.20160510
    
Protein Phosphatase 2CInvolved in the Flagellar Transition Zone Structure of Chlamydomonas reinhardtii
HE Wei, PAN Jun-min
College of Life Science, Tsinghua University, Beijing 100084, China
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Abstract  

Objective:Using insertional mutagenesis in Chlamydomonas to identify genes required for flagellar assembly, so as to researching the function of the gene related to flagellar assembly. Methods:By transforming the DNA fragment harboring paromomycinresistance gene into Chlamydomonas randomly, the mutant strainwith flagellar defect is obtained and identified the disrupted gene was CrPP2C(Chlamydomonas reinhardtii type 2C protein phosphatase).Then the CrPP2C gene function is analyzed by biochemistry methods. Results:Theflagella abnormal mutantsthat show short flagella or none flagella are obtained by electroporation transformation.DNA sequence analysis show that the CrPP2C gene is disrupted. Transformation of mutants with CrPP2C-HA could rescue the flagellar phenotype.The mutants could regenerateflagella to the original length and shorte normally. Electron microscopyimages show incomplete Y-link structure in the transition zone of flagella. Conclusion:The CrPP2C gene is associated with the transition zone structure and affects the assembly of flagella.



Key wordsFlagellar transition zone      Chlamydomonas reinhardtii      CrPP2C     
Received: 19 February 2016      Published: 25 May 2016
ZTFLH:  Q819  
Cite this article:

HE Wei, PAN Jun-min. Protein Phosphatase 2CInvolved in the Flagellar Transition Zone Structure of Chlamydomonas reinhardtii. China Biotechnology, 2016, 36(5): 68-73.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160510     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I5/68

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