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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (10): 113-120    DOI:
    
A Review on Analytic Techniques of Fibrinolytic Protein
HUANG Ming-xing, YE Yun, HAN Ya-li
Department of Biological Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China
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Abstract  

PAs are serine proteases of tryptic specificity which can convert the proenzyme, plasminogen, into an active enzyme plasmin. For the assay of PAs activity, four techniques have been established. Fibrin plate method and colorimetric assay using chromogenic substrates are two early methods which can be used for semi-quantitative analysis. Reverse fibrin autography and fibrin zymography are two new qualitative techniques which can be used to detect the molecular weight or isoelectric point of unknown PAs. The developing process and principle of these four techniques were reviewed, and the potential applications, advantages and disadvantages were discussed.



Key wordsFibrinolytic protein      Fibrin plate method      Colorimetric assay using chromogenic substrates      Reverse fibrin autography      Fibrin zymography     
Received: 08 November 2010      Published: 25 October 2011
ZTFLH:  Q819  
Cite this article:

HUANG Ming-xing, YE Yun, HAN Ya-li. A Review on Analytic Techniques of Fibrinolytic Protein. China Biotechnology, 2011, 31(10): 113-120.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I10/113


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