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荧光假单胞菌天冬氨酸转氨酶的基因克隆及其在大肠杆菌中的表达 |
邵楠1,王虹2,李荣贵2 |
1. 浙江大学竺可桢学院
2. 青岛大学生物系 |
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Cloning and Expression of Aspartate Aminotransferase from Pseudomonas fluorescens in Escherichia coli |
[1] 严明,许伟, 陈菲. E.coli天冬氨酸转氨酶结构与功能关系的研究进展. 生物加工过程,2004,2(3):14~18
Yan M, Xu W, Chen F. Chinese Journal of Bioprocess Engineering, 2004,2(3): 14~18
[2] 荣墨克,谢风, 孙荣武,等. 人心肌胞质天门冬氨酸转氨酶的纯化及性质. 生物化学与生物物理进展, 1991, l8(1):56~59
Rong M K, Xie F, Sun R W, et al. Progress in Biochemistry and Biophysics, 1991, l8(1): 56~59
[3] 宫长斌,徐娴,李霜,等. 组成型天冬氨酸转氨酶基因工程菌的构建与高效表达.过程工程学报,2007,7(3):574~578
Gong C B,Xu X, Li S, et al. Chinese Journal of Process of Engineering,2007,7(3):574~578
[4] Fotheringham I G, Dacey S A, Taylor P P, et al. The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes. Biochemistry Journal, 1986, 234(3): 593~604
[5] Sung M H, Tanizawa K, Tanaka H, et al. Thermostable aspartate aminotransferase from a thermophilic Bacillus species. Gene cloning, sequence determination, and preliminary x-ray characterization. Journal of Biological Chemistry, 1991, 26(4):2567~2572
[6] Schultz C J, Corruzi G M. The aspartate aminotransferase gene family of Arabidopsis encodes isoenzymes localized to three distinct subcellular compartments. Plant Journal, 1995, 7(1): 61~75
[7] 郑维,权春善,朴永哲,等. 一种快速提取细菌总DNA的方法研究.中国生物工程杂志,2006,26(4):75~80
Zheng W, Quan C S, Piao Y Z,et al. China Biotechnology,2006, 26(4):75~80
[8] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72: 248~254
[9] 杨昌国,许 叶,江摩海,等. 转氨酶测定标准化的意见.江西医学检验,1993,11(3):24~28
Yang C G, Xu Y, Jiang M H, et al. Jiangxi Journal of Medical Laboratory Sciences, 1993,11(3):24~28
[10] Wilkie S E, Warren M J. Recombinant expression, purification, and characterization of three isoenzymes of aspartate aminotransferase from Arabidopsis thaliana. Protein Expression and Purification,1998, 12(3):381~389
[11] Bartsch K, Schneiderr R, Schultz A. Stereospecific production of the herbicide phosphinothricin (Glufosinate): purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process. Applied and Environmental Microbiology, 1996,62(10): 3794~3799
[12] Nagashima F, Tanase S, Fukumoto Y, et al. cDNA cloning and expression of pig cytosolic aspartate aminotransferase in Escherichia coli: aminoterminal heterogeneity of expressed products and lack of its correlation with enzyme function. Biochemistry, 1989, 28(3):1153~1160
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