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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2014, Vol. 34 Issue (06): 90-97    DOI: 10.13523/j.cb.20140613
综述     
丁醇基因在大肠杆菌中表达的现状与展望
王庆龙1,2, 刘莉2, 史吉平2, 薛永常1, 孙俊松2
1. 大连工业大学生物工程学院 大连 116034;
2. 中国科学院上海高等研究院生物炼制实验室 上海 201210
Current Status and Prospects of the Expression of Butanol Pathway in Escherichia coli
WANG Qing-long1,2, LIU li2, SHI Ji-ping2, XUE Yong-chang1, SUN Jun-song2
1. School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China;
2. Biorefinery Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
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摘要：

随着化石能源过度开采带来的能源短缺与环境恶化，丁醇凭借着其优越的理化性质成为了最具潜力的绿色燃料之一。近几年微生物在生物能源生产研究中受到广泛关注，主要集中在梭菌丁醇合成途径的异源表达。目前利用大肠杆菌产丁醇的产量已经接近产丁醇的天然菌株的产量。然而，大肠杆菌产丁醇仍存在很多限制性因素。主要从乙酰辅酶A依赖途径评述大肠杆菌生产丁醇的限制因素，并讨论提高丁醇产量需要解决的问题。
关键词： 大肠杆菌;  生物丁醇;  丁醇代谢    
Abstract:

Biobutanol is regarded as a promising biofuel amid the energy crisis and global problems brought by excess use of fossil energy. In recent years, microorganisms including Escherichia coli are of interests in bioengineering studies for bioproduction of butanol, mostly done by heterologous expression of clostridia derived synthetic butanol pathway. Currently, the maximal yield of butanol production reported in Escherichia coli has been close to that in natural strains; however, there still existed many problems daunting the attempts to further improve the biobutanol level by using this microorganism. The engineered metabolic pathways for formation of n-butanol in E. coli were briefly summarized, the limiting factors existed in bioengineering studies were analyzed, and the possible solutions that might help in further improvement of biobutanol productivity using E. coli as host strain were shared.
Key words: Butanol metabolism    Escherichia coli    Biobutanol
收稿日期: 2014-03-12 出版日期: 2014-06-25
ZTFLH:  Q786  
基金资助:

国家自然科学基金资助项目（31270122）
通讯作者: 薛永常, 孙俊松     E-mail: xueych@dlpu.edu.cn;sunjs@sari.ac.cn
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引用本文:

王庆龙, 刘莉, 史吉平, 薛永常, 孙俊松. 丁醇基因在大肠杆菌中表达的现状与展望[J]. 中国生物工程杂志, 2014, 34(06): 90-97.

WANG Qing-long, LIU li, SHI Ji-ping, XUE Yong-chang, SUN Jun-song. Current Status and Prospects of the Expression of Butanol Pathway in Escherichia coli. China Biotechnology, 2014, 34(06): 90-97.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140613    或    https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I06/90


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