海藻酸转化生物乙醇研究进展

中国生物工程杂志

2013, Vol. 33

Issue (1): 122-127

综述

海藻酸转化生物乙醇研究进展

钱龙¹, 唐丽薇¹, 黄庶识², 伊日布斯¹

1. 昆明理工大学生命科学与技术学院生物转化研究室昆明 650500;
2. 广西科学院南宁 530007

Research Progress of Bioethanol from Alginate Fermentation

QIAN Long¹, TANG Li-wei¹, HUANG Shu-shi², Chagan Irbis¹

1. Laboratory of Bioconvertion, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China;
2. Guangxi Academy of Sciences, Nanning 530007, China

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摘要： 作为第三代生物燃料,大型褐藻类生物质转化燃料乙醇的研究受到广泛的关注。但是,现有的乙醇工业菌株并不能利用褐藻中的主要成分海藻酸,这个问题是海藻生物乙醇实现工业化生产的主要技术难关。近几年随着对海藻酸裂解酶和海藻酸降解菌代谢途径的深入研究,科研人员构建了不同的海藻酸发酵菌株,为高效转化大型海藻生产生物乙醇提供了可行的技术基础。这篇文章对海藻酸资源概况和海藻酸转化生物乙醇存在的科学问题及其研究进展进行了综述。

关键词： 海藻酸; 海藻酸裂解酶; 海藻酸代谢途径; 第三代生物乙醇

Abstract: As the third-generation biofuel, the bioethanol from macroalgae biomass fermentation have received widespread attention. However, the present ethanol industry strains were not able to utilize alginate that is the main ingredient in seaweed. This is one of the major technical difficulties to impede to achieve the industrial production of alginate bio-ethanol. In recent years, the cleavage enzyme and alginate degrading bacteria metabolic pathways have been studied in depth. The researchers constructed different alginate fermentation strains, and provided a viable technological support for the efficient conversion from alginate to bio-ethanol. This article reviewed resource profile of alginate and the scientific issues of bioethanol production by fermentation with alginate.

Key words: Alginate Alginate lyase Alginate metabolic pathways The third-generation biofuel

收稿日期: 2012-11-02 出版日期: 2013-01-25

ZTFLH:

Q815

基金资助: 广西自然科学基金重点资助项目(2010GXNSFD013029)

通讯作者: 伊日布斯 E-mail: irbisc@gmail.com

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引用本文:

钱龙, 唐丽薇, 黄庶识, 伊日布斯. 海藻酸转化生物乙醇研究进展[J]. 中国生物工程杂志, 2013, 33(1): 122-127.

QIAN Long, TANG Li-wei, HUANG Shu-shi, Chagan Irbis. Research Progress of Bioethanol from Alginate Fermentation. China Biotechnology, 2013, 33(1): 122-127.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I1/122

[1] Rajeendernath P, Donald J B. Composition and block structure of alginates from New Zealand brown seaweeds. Carbohydrate Research, 1996, 293(1):119-132.
[2] 纪明侯, 张燕霞. 我国经济褐藻的化学成分研究. 海洋与湖沼, 1962, 4(3-4):161-167. Ji M H, Zhang Y X, Study on the chemical composition of the chinese economi biown seaweeals, Oceanologia Etlimnologin Sinica, 1962, 4(3-4):161-167.
[3] 张菊清.大型海藻的经济价值. 舟山师专学报, 1998, 1:76-79. Zhang J Q. The economic value of seaweed. Joarnal of Zhoushan Nornal School, 1998, 1:76-79.
[4] Roesijadi G, Jones S B, Snowden-Swan L J, et al. Macroalgae as a biomass feedstock: a preliminary analysis(R). Washington: Pacific Northwest National Laboratory, 2010.
[5] 范晓, 韩丽君, 周天成, 等. 中国沿海经济海藻化学成份的测定. 海洋与湖沼, 1995, 26(2):199-207. Fan X, Han L J, Zhou T C, et al. Chemical compesition ofecoromic seaweats from the coast of china. Oceandogia Etlimologia Sinica, 1995, 26(2):199-207.
[6] Ge L L, Wang P, Mou H J.Study on saccharification techniques of seaweed wastes for the transformation of ethanol. Renewable Energy, 2011, 36(1):84-89.
[7] Haug A, Larsen B, Smidsrød O. Studies on the sequence of uronic acid residues in alginic acid. Acta Chemica Scandinavica, 1967, 21:691-704.
[8] Park H H, Kam N, Lee E Y, et al. Saccharification of alginate by using exolytic oligoalginate lyase from marine bacterium Sphingomonas sp. MJ-3. Journal of Industrial and Engineering Chemistry, 2011, 17(5-6):853-858.
[9] Lin T Y, Hass W Z. Pathway of alginic acid synthesis in the marine brown alga, Fucus gardneri Silva. J Biol Chem, 1966, 241(22):5284-5297.
[10] Rahman M M, Inoue A, Tanaka H. Isolation and characterization of two alginate lyase isozymes, AkAly28 and AkAly33, from the common sea hare Aplysia kurodai. Comparative Biochemistry and Physiology, Part B. 2010, 157(4):317-325.
[11] Lundqvist L C, Jam M, Barbeyron T, et al. Substrate specificity of the recombinant alginate lyase from the marine bacteria Pseudomonas alginovora. 2012, 352:44-50.
[12] Díaz-Barrera A, Soto E, Altamirano C. Alginate production and alg8 gene expression by Azotobacter vinelandii in continuous cultures. Journal of Industrial Microbiology & Biotechnology, 2012, 39(4):613-621.
[13] Li J W, Dong S, Song J. Purification and characterization of a bifunctional alginate lyase from Pseudoalteromonas sp. SM0524. Marine Drugs, 2011, 9(1):109-123.
[14] Hamza A, Piao Y L, Kim M S, et al. Insight into the binding of the wild type and mutated alginate lyase (AlyVI) with its substrate: A computational and experimental study. Biochimica Biophysica Acta (BBA) - Proteins and Proteomics, 2011,1814(12):1739-1747.
[15] In Lee S, Choi S H, Lee E Y, et al. Molecular cloning, purification, and characterization of a novel polyMG-specific alginate lyase responsible for alginate MG block degradation in Stenotrophomas maltophilia KJ-2. Appl Microbiol Biotechnol, 2012, 95(6):1643-1653.
[16] Rahman M M, Inoue A, Tanaka H, et al. cDNA cloning of an alginate lyase from a marine gastropod Aplysia kurodai and assessment of catalytically important residues of this enzyme. Biochimie. 2011,93(10):1720-1730.
[17] Rahman M M, Wang L, Inoue A, et al. cDNA cloning and bacterial expression of a PL-14 alginate lyase from a herbivorous marine snail Littorina brevicula. Carbohydr Res. 2012, 360:69-77.
[18] Kim H T, Chung J H, Wang D, et al. Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans 2-40. Appl Microbiol Biotechnol, 2012 Mar, 93(5):2233-2239.
[19] Schaumann K, Weide G. Enzymatic degradation of alginate by marine fungi. Hydrobiologia, 1990, 204-205(1): 589-596.
[20] Wong T Y, Preston L A, Schiller N L. Alginate lyase: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications. The Annual Review of Microbiology. 2000, 54:289-340.
[21] Uchimura K, Miyazaki M, Nogi Y,et al. Cloning and Sequencing of Alginate Lyase Genes from Deep-Sea Strains of Vibrio and Agarivorans and Characterization of a New Vibrio Enzyme. Mar Biotechnol (NY). 2010,12(5):526-533.
[22] Li J W, Dong S, Song J, et al. Purification and Characterization of a Bifunctional Alginate Lyase from Pseudoalteromonas sp. SM0524. Marine Drugs. 2011, 9(1):109-123.
[23] Schiller N L, Monday S R, Boyd C M, et al. Characterization of the Pseudomonas aeruginosa Alginate Lyase Gene (algL): Cloning, Sequencing, and Expression in Escherichia coli. J Bacteriol. 1993, 175(15):4780-4789.
[24] Duan G, Han F, Yu W. Cloning, sequence analysis, and expression of gene alyPI encoding an alginate lyase from marine bacterium Pseudoalteromonas sp. CY24 2009. Can J Microbiol. 2009,55(9):1113-1118.
[25] Hisano T, Yonemoto Y, Yamashita T. Direct uptake of alginate molecules through a pit on the bacterial cell surface: a novel mechanism for the uptake of macromolecules. Journal of Fermentation and Bioengineering, 1995, 79(6):538-544.
[26] Hashimoto W, He J, Wada Y, et al. Proteomics-based identification of outer-membrane proteins responsible for import of macromolecules in Sphingomonas sp. A1: alginate-binding flagellin on the cell surface. Biochemistry, 2005, 44(42):13783-13794.
[27] Momma K, Okamoto M, Mishima Y, et al. Direct evidence for Sphingomonas sp. A1 periplasmic proteins as macromolecule-binding proteins associated with ABC transporter: molecular insights into alginate transport in the periplasm. Biochemistry, 2005, 44(13):5053-5064.
[28] Momma K, Okamoto M, Mishima Y, et al. A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules. Journal of Bacteriology, 2000, 182(4):3998-4004.
[29] Yoon H J, Hashimoto W, Miyake O, et al. Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyases. Protein Expr Purif, 2000, 19(1):84-90.
[30] Hashimoto W, Miyake O, Momma K, et al. Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J Bacteriol, 2000, 182 (16):4572-4577.
[31] Takase R, Ochiai A, Mikami B, et al. Molecular identification of unsaturated uronate reductase prerequisite for alginate metabolism in Sphingomonas sp. A1. Biochimica Biophysica Acta, 2010, 1804(9):1925-1936.
[32] Wargacki A J, Leonard E, Win M N,et al. An engineered microbial platform for direct biofuel production from brown macroalgae. Science, 2012, 335(6066):308-313.
[33] Horn S J, Aasen I M. Ethanol production from seaweed extract. Journal of Industrial Microbiology & Biotechnology, 2000, 25(5):249-254.
[34] Adams J M, Gallagher J A, Donnison I S. Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments. Journal of Applied Phycology, 2009, 21(5):569-574.
[35] Takeda H, Yoneyama F, Kawai S, et al. Bioethanol production from marine biomass alginate by metabolically engineered bacteria. Energy & Environmental Science, 2011, 7:2575-2581.

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