OsRacD是光敏核不育水稻农垦58S光周期育性转换信号通路中的重要分子开关，通过酵母双杂交筛选，从水稻幼穗中分离到一种与OsRacD 互作的新的肌球蛋白重链编码基因的cDNA片段，命名为OsMY1。本文在酵母双杂交体系鉴定的基础上，构建了OsMY1和OsRacD的原核表达载体，表达和纯化了融合蛋白GST-OsMY1 和His6-OsRacD, 通过pull down实验进一步确认了上述互作关系。本研究提示OsMY1和OsRacD可能在功能上相关，为深入研究二者在水稻生理功能上的联系提供了重要线索和依据。
OsRacD is a critical molecular switch involved in photoperiod fertility conversion of photoperiod sensitive genic male sterile rice. Taking OsRacD as bait, a new rice myosin heavy chain partial cDNA was isolated from the rice panicle by yeast two-hybrid,and designated OsMY1.In this study, the interaction between OsMY1 and OsRacD had been testified by yeast two-hybrid and pull down assay, the results showed that OsMY1 could bind with OsRacD, Our study suggested that OsMY1 maybe the target of OsRacD. The results also provided important evidence for further study on the functional relationship between OsMY1 and OsRacD in vivo.
侯成千,梁卫红,王军. 水稻OsMY1和OsRacD基因互作的分子鉴定[J]. 中国生物工程杂志, 2008, 28(7): 63-66.
Liang Wei－Hong . Identification of the Genetic Interaction between OsMY1 and OsRacD from Oryza sativa, L. China Biotechnology, 2008, 28(7): 63-66.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I7/63
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