猪胞浆内单精子注射研究进展

中国生物工程杂志

2009, Vol. 29

Issue (02): 108-113

综述

猪胞浆内单精子注射研究进展

张廷宇,马恒东

四川农业大学动物科技学院

Advances in Porcine Intracytoplasmic Sperm Injection

全文: PDF(413 KB) HTML

摘要： 胞浆内单精子注射将体外受精和显微操作技术相结合，已成功应用于哺乳动物受精机理研究，家畜性控胚胎生产，动物转基因等领域。综述了猪胞浆内单精子注射的主要影响因素，包括卵母细胞体外成熟和预处理、精子的选择和处理、注射卵的人工激活以及操作技术的改进，为进一步提高猪胞浆内单精子注射的效率以及该技术的应用提供参考。

关键词： 猪;ICSI;预处理;激活

Abstract:

Intracytoplasmic sperm injection, which combined with in vitro fertilization and micromanipulation techniques, has been applied to research the molecular mechanisms of fertilization, and produce the sexing livestock and transgenic animals. The review described the research advances in porcine intracytoplasmic sperm injection, including in vitro maturation and pretreatment of oocytes, selection and treatment of spermatozoon, artificial activation of injected oocytes after injection, and improvement of the operation technical.

收稿日期: 2008-09-01 出版日期: 2009-03-31

通讯作者: 马恒东

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引用本文:

张廷宇,马恒东. 猪胞浆内单精子注射研究进展[J]. 中国生物工程杂志, 2009, 29(02): 108-113.

ZHANG Ting-Yu- Ma-Heng-Dong. Advances in Porcine Intracytoplasmic Sperm Injection. China Biotechnology, 2009, 29(02): 108-113.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I02/108

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