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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (09): 1-6    DOI: Q2
    
Tumor necrosis factor-a-induced expression of heat shock protein 70 in Neonatal rat ventricular cardiomyocytes
HUANG Yin-xia,WEI Ying-jie,CUI Chuan-jue,ZHANG Xiao-ling,LI Jun
Key Laboratory of Cardiovascular Regenerative Medicine, Ministry of Health, Cardiovascular Institute and Fu-Wai Hospital, PUMC and CAMS, Beijing 100037, China  
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Abstract  

Heat shock protein 70 is essential for cellular recovery, survival and maintenance of cellular function. Heat shock protein 70 function as molecular chaperons in protein folding and it is a role of cardioprotection against various stresses. We have studied the different proteins expression of tissues from failing hearts using proteomics before. We found that HSP70 was highly expressed in the tissues and blood samples from heart failure patients compaired with non-failing hearts. For exploiting the use of HSP70 as a therapeutic marker, we sthudy the HSP70 expression using Neonatal rat ventricular cardiomyocytes(NRVM) by teating with TNF-a with various concentrations and time points in vitro. Primary cultures were prepared from neonatal rat ventricular myocytes. Cultured cardiomyocytes were incubated with TNF-a at doses of 40, 200 and 800U /ml for a dynamic time point as 1, 6, 12 and 24 hours. NRVM treated with heat shock(42℃) were used as positive control. The expression of myocyte HSP70 was quantified by Western blot analysis and immunohistochemistry using a specific monoclonal antibody. Immunohistochemical analysis revealed HSP70 were nearly not visible in control cells. However, at 12 h post-hypoxia/SD, HSP70-immunoreactivity (IR) was evident in the cells. These studies constitute the initial demonstration that TNF-alpha exerts concentration- and time-dependent effects on the expression of HSP 70 in NRVM. The expression of HSP70 influenced by TNF-a can indicate the possibility of a pharmacological approach to HSP70 induction and cardiac protection, which may ultimately be of clinical relevance.



Key wordsHeat shock protein 70      Hypoxia/Serum Deprivation      TNF-α      Neonatal rat ventricular cardiomyocytes(NRVM)     
Received: 21 December 2009      Published: 25 August 2010
Cite this article:

HUANG Yin-Xia, WEI Yang-Jie, CUI Chuan-Jue, ZHANG Xiao-Ling, LI Jun. Tumor necrosis factor-a-induced expression of heat shock protein 70 in Neonatal rat ventricular cardiomyocytes. China Biotechnology, 2010, 30(09): 1-6.

URL:

https://manu60.magtech.com.cn/biotech/Q2     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I09/1

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