Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (03): 80-84    DOI:
    
Prokaryotic Expression and Characteristics Reseach of Pinellia Ternate and Pinellia Pedatisecta Lectins
Download: HTML   PDF(708KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Pinellia ternate and Pinellia pedatisecta lectins with biological functions could be obtained through procaryon expression. Their biological similarities and differences were analyzed and further experiments were conducted to discuss the agglutinative activity difference between polymer lectin and metamer lectin. The results showed that agglutinative activity of Pinellia pedatisecta was four times more than that of Pinellia ternate. The differences of the agglutinative activity and pharmacologic action were mainly because two site mutations of Pinellia pedatisecta in the third active site compared with the amino acids sequence in Pinellia ternate. In addition, the metamer lectin expressed by prokaryotic expression system did not agglutinate rabbit red blood cells. This research conduct further discussion about the difference between two lectins and the difference between polymer lectin and metamer lectin. It would be applicable for solving the problem of lacking Pinellia resources.



Key wordsPinellia ternate;Pinellia pedatisecta;lectin;prokaryotic expression;hemagglutinating activity     
Received: 08 October 2008      Published: 31 March 2009
ZTFLH:  Q786  
Cite this article:

LIANG Jiang-Li- Chen-Bei- Tian-Xiao-Beng- Liu-Xiao-Chuan. Prokaryotic Expression and Characteristics Reseach of Pinellia Ternate and Pinellia Pedatisecta Lectins. China Biotechnology, 2009, 29(03): 80-84.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I03/80

[1] 金银兵, 杨宗岐, 陈集双. 三叶半夏组培快繁与优良单株的选育. 中国生物工程杂志, 2008, 28 (6): 48~54 Jin Y B, Yang Z Q, Chen J S. China Biotechnology, 2008, 28 (6): 48~54 [2] 毛子成, 彭正松. 半夏研究进展. 江西科学, 2002, (1): 193~195 Mao Z C, Peng ZH S. Jiangxi Science, 2002, (1): 193~195 [3] 洪行球, 沃兴德, 何一中, 等. 半夏降血脂作用研究. 浙江中医学院学报, 1995, 19 (2): 28~29 Hong X Q, Wo X D, He Y Z, et al. Journal of Zhejiang College of TCM, 1995, 19 (2): 28~29 [4] 陈惠玲, 宋锦芬, 陶宗晋. 半夏蛋白的抗兔胚泡着床作用. 生理学报, 1984, 34 (4): 388~389 Chen H L, Song J F, Tao Z J. Acta Physiologica Sinica, 1984, 34 (4): 388~389 [5] 陶宗晋, 徐琴钰, 吴克佐, 等. 半夏蛋白的分离、结晶、生物活力和一些化学性质. 生物化学和生物物理学报, 1981, 13 (1): 77~82 Tao Z J, Xu Q Y, Wu K Z, et al. Acta Biochimica et Biophysica Sinica, 1981, 13 (1): 77~82 [6] 朱铭伟, 周抗美, 丁声颂. 掌叶半夏总蛋白对卵巢癌细胞株继任脐血造血细胞的作用. 上海医科大学学报, 1999, 26 (6): 455~458 Zhu M W, Zhou K M, Ding S S. J Shanghai Med Univ, 1999, 26(6): 455~458 [7] 孙册, 徐继华, 翟世康, 等. 半夏蛋白的若干生物学性质. 生物化学与生物物学报, 1983, 15 (4): 334~338 Sun C, Xu J H, Zhai S K, et al. Acta Biochimica et Biophysica Sinica, 1983, 15(4): 333~338 [8] 王志斌, 李学勇, 郭三堆. 植物凝集素与抗虫基因工程. 生物技术通报, 1998, 2 5~10 Wang Z B, Li X Y, Guo S D. Bulletin of Biotechnology, 1998, 2: 5~10 [9] Yao J H, Sun X F, Tang K X. Molecular cloning of lectin gene from Pinellia ternata. Journal of Fudan University, 2001, 40 (4): 461~464 [10] Lin J, Zhou X, Gao S, et al. cDNA cloning and expression analysis of a mannosebinding lectin from Pinellia pedatisecta. Journal of Biosciences, 2007, 32 (2): 241~249 [11] Konozy E H E, Bernardes E S, Rosa C, et al. Isolation, purification, and physicochemical characterization of a Dgalactosebinding lectin from seeds of Erythrina speciosa. Archives of Biochemistry and Biophysics, 2003, 410 (2): 222~229 [12] Martinez Cruz M, Zenteno E, Cordoba F. Purification and characterization of a galactosespecific lectin from corn (Zea mays) coleoptyle. Biochim Biophy Acta, 2001, 1568 (1): 37~44 [13] Wang H, Ng T B, Liu Q. Isolation of a new heterodimeric lectin with mitogenic activity from fruiting bodies of the mushroom Agrocybe cylindracea. Life Sciences, 2002, 70 877~885 [14] Lin J, Zhou X, Fei J, et al. Genomic cloning and characterization of a PPA gene encoding a mannosebinding lectin from Pinellia pedatisecta. Biocell, 2006, 30 (1): 15~25 [15] 王克夷, 郭锰. 半夏凝集素的分离纯化和在植物中的分布. 生物化学杂志, 1993, 9 (5): 544~548 Wang K Y, Guo M. Chinese Biochemical Journal, 1993, 9 (5): 544~548 [16] 范美华, 周吉源. 半夏的研究进展. 西北药学杂志, 2004, 19 (2): 90~93 Fan M H, Zhou J Y. Northwest Pharmaceutical Journal, 2004, 19 (2): 90~93

No related articles found!