Research Progress in Improving the Yield of Butenyl-spinosyn Based on Metabolic Pathway Optimization

doi:10.13523/j.cb.2212039

China Biotechnology

2023, Vol. 43

Issue (7): 122-135 DOI: 10.13523/j.cb.2212039

Research Progress in Improving the Yield of Butenyl-spinosyn Based on Metabolic Pathway Optimization

Yu XIE^1,²,Bo SUN²,Jia SONG²,Chen ZHAO^2,^**(

),Wan-zhong ZHANG^1,^**(

)

1 College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China
2 Academy of National Food and Strategic Reserves Administration, Beijing 100037, China

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Abstract

Butenyl-spinosyn, a macrolide antibiotic produced by Saccharopolyspora pogona, is a green, environmentally-friendly novel bio-pesticide with high application value in pest control and grain storage. However, the low yield of the wild-type strain has limited the application of this compound; therefore, high-yield strain breeding becomes a question that needs to be solved urgently. In this paper, new ideas of genetic breeding for increasing butenyl-spinosyn in recent years were summarized. The studies on increasing butenyl-spinosyn yield were reviewed, based on the modification of carbon, nitrogen and phosphate metabolic pathways as well as transcriptional regulation of S.pogona, and the prospect of strain breeding through heterologous expression was also discussed, in order to provide effective reference for the construction and optimization of butenyl-spinosyn high yielding strains.

Key words： Butenyl-spinosyn Saccharopolyspora pogona Biological pesticide High-yield strain Metabolic pathway optimization

Received: 27 December 2022 Published: 03 August 2023

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	Yu XIE
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Cite this article:

Yu XIE, Bo SUN, Jia SONG, Chen ZHAO, Wan-zhong ZHANG. Research Progress in Improving the Yield of Butenyl-spinosyn Based on Metabolic Pathway Optimization. China Biotechnology, 2023, 43(7): 122-135.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2212039 OR https://manu60.magtech.com.cn/biotech/Y2023/V43/I7/122

Fig.1 Structural formula of spinosyn and butenyl-spinosyn (a) Spinosyn (b) Butenyl-spinosyn

Table 1 Insecticidal spectrum of butenyl-spinosyn

Fig.2 Comparison of butenyl-spinosyn and spinosyn biosynthetic gene clusters

Fig.3 Module structure of spinosyn and butenyl-spinosyn polyketide synthase

Fig.4 The biosynthetic pathway of butenyl-spinosyn

Fig.5 Metabolic network of butenyl-spinosyn

Table 2 Effects of the optimization of carbon metabolism pathway on the production of spinosad and butenyl-spinosyn

Table 3 Increase the production of butenyl-spinosyn through transcriptional regulation

Table 4 Heterologous host optimization


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