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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (8): 9-15    DOI: 10.13523/j.cb.20150802
    
Comparative Analysis of Two Rotavirus VP4 Epitopes Inserted on the Same Site of VP6 Vector Protein
XIA Wen-yue1, WANG Jing1, ZHAO Bing-xin1, PAN Xiao-xia2, WEN Yu-ling1, CHEN Yuan-ding1
1. Chinese Academy of Medical Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Kunming 650118, China;
2. Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University, Kunming 650500, China
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Abstract  

Objective: To evaluate immunoreactivity and value of chimeric proteins that carry VP4 epitopes on the same insertion site using rotavirus VP6 as a vector. Methods: Two plasmids that carry genes of chimeric proteins carrying VP4 epitopes on the same insertion site using rotavirus VP6 as a vector were constructed by gene cloning and recombination; the chimeric proteins were expressed in E. coli cells, detected with anti-VP6 antibodies, as antigens inoculated in guinea pigs; and neutralization activities of antibodies in sera from chimeric proteins inoculated guinea pigs were determined. Results: the expressed chimeric proteins could react with antibodies against VP6 of rotavirus, could elicit production of antibodies in inoculated guinea pigs that reacted with vector protein VP6F, VP6 and VP4 of strain Wa virus, neutralized infectivities of Wa virus in MA104 cell. The results showed that chimeric proteins carrying different VP4 epitopes on the same insertion site retained its original backbone structure stability and had good immunoreactivity; results obtained are valuable for development of novel rotavirus vaccines.



Key wordsRotavirus      Epitopes      Epitope presenting vector      Recombinant chimeric protein      Immunoractivity     
Received: 15 April 2015      Published: 25 August 2015
ZTFLH:  Q789  
Cite this article:

XIA Wen-yue, WANG Jing, ZHAO Bing-xin, PAN Xiao-xia, WEN Yu-ling, CHEN Yuan-ding . Comparative Analysis of Two Rotavirus VP4 Epitopes Inserted on the Same Site of VP6 Vector Protein. China Biotechnology, 2015, 35(8): 9-15.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150802     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I8/9


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