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| Artificial sRNAs Silencing csrA to Optimize the Production of L-tyrosine in Escherichia coli |
| YAO Yuan-feng, ZHAO Ying, ZHAO Guang-rong |
| Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China |
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Abstract As a powerful gene regulation tool, small regulatory RNAs (sRNAs) have been widely applied in animals and plants. Recently, with the continual founding of different sRNAs and the understanding about their regulatory mechanisms in bacteria, the new developed artificial sRNAs engineering also shows a huge advantage on the microbial metabolic engineering. Carbon storage regulator (CsrA) is a post-transcriptional global regulator that has a negative role in the L-tyrosine biosynthetic pathway. By designing and screening sRNAs sequence of csrA, its effect on the L-tyrosine production in E. coli was analyzed. The results indicated that the artificial sRNAs could lead to remarkable increase of L-tyrosine production. High copy number expression of shorter anti-csrA sRNA2 which improved 1.2-fold L-tyrosine production was better than the longer sRNA1. This new strategy, being simple yet very powerful for global metabolic regulation, is thus expected to facilitate the efficient development of synthetic biology and microbial cell factories.
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Received: 02 May 2013
Published: 25 August 2013
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