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| Purification and Immunologic Study of Subunit Vaccines 3STaM(G)-K99 and 3STaM(S)-K99 with Tandem Copies of Mutant Heat-stable Enterotoxin STa and Colonization Factor K99 from Enterotoxigenic Escherichia coli |
| GUO Le1,2, LIU Kun-mei2, LI Min1, ZHAO Hui2, XU Guang-xian2, DUAN Xiang-guo2, HAN Xue-bo2, YANG Hua1, WANG Yu-jiong1 |
1. Key Laboratory of Ministry of Education for the Conservation and Utilization of Special Biological Resource of Western China, College of Life Science, Ningxia University, Yinchuan 750021, China;
2. Ningxia Institute of Laboratory Medicine, School of Laboratory Medicine, Ningxia Medical University, Yinchuan 750004, China |
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Abstract Enterotoxigenic Escherichia coli (ETEC) continue to be a significant cause of diarrheal disease in newborn calves and piglets. The main virulence factors of ETEC include colonization factors (CFs), heat-labile (LT) and heat-stable (ST) enterotoxins. In previous study, two subunit vaccines with tandem copies of mutated heat-stable enterotoxin (STaM) and colonization factor K99, named 3STaM(G)-K99 and 3STaM(S)-K99, were constructed and expressed in Escherichia coli. The fusion proteins 3STaM (G)-K99 and 3STaM(S)-K99 were purified by anion-exchange chromatography and then their immunological features and ability to induce neutralizing antibodies against natural STa were evaluated in rabbit and mouse models. The experimental results indicated that the subunit vaccines 3STaM(G)-K99 and 3STaM(S)-K99 could induce medium levels of specific antibodies against natural STa, ETEC or the fusion protein STa-K99 with unmutated STa and K99. Besides, the enterotoxicity of STaM components in 3STaM(G)-K99 and 3STaM(S)-K99 were dramatically reduced, and the 3STaM(G)-K99 and 3STaM(S)-K99 vaccines could induce neutralizing antibodies which showed effectively inhibitory effect on the enterotoxicity of natural STa. The subunit vaccines 3STaM(G)-K99 and 3STaM(S)-K99 are promising molecules to be investigated as ETEC vaccine antigen candidate.
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Received: 01 April 2013
Published: 25 July 2013
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| Fund: This work was supported by the National Natural Science Foundation of China (JKY30560110) |
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Cite this article:
GUO Le, LIU Kun-mei, LI Min, ZHAO Hui, XU Guang-xian, DUAN Xiang-guo, HAN Xue-bo, YANG Hua, WANG Yu-jiong. Purification and Immunologic Study of Subunit Vaccines 3STaM(G)-K99 and 3STaM(S)-K99 with Tandem Copies of Mutant Heat-stable Enterotoxin STa and Colonization Factor K99 from Enterotoxigenic Escherichia coli. China Biotechnology, 2013, 33(7): 18-24.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2013/V33/I7/18
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