Safety Evaluation of <em>Bacillus amyloliquefaciens</em> Microcapsules Used for Aquaculture

China Biotechnology

2012, Vol. 32

Issue (05): 58-65 DOI:

Safety Evaluation of Bacillus amyloliquefaciens Microcapsules Used for Aquaculture

CAO Hai-peng¹, WEI Ruo-peng², HE Shan³, LU Li-qun¹

1. National Pathogen Collection Centre, Shanghai Ocean University, Shanghai 201306, China;
2. Shanxi Veterinary Drug and Feed Engineering Technology Research Center, Yuncheng 044000, China;
3. College of Education Shanghai Normal University, Shanghai 200234, China

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Abstract

Objective: The present study was to evaluate the safety of Bacillus amyloliquefaciens microcapsules used for aquaculture. Methods: According to Chemicals—Alga growth inhibition test (GB/T21805-2008), Water quality—Determination of the acute toxicity of substance to Daphnia (Daphnia magna straus) (GB/T13266-91), Water quality—Determination of the acute toxicity of substance to freshwater fish (Brachydanio rerio Hamilton-Buchanan) (GB/T13267-91) and Clinical experiment technical practice for fishery drugs, the growth inhibition of the B. amyloliquefaciens microcapsule on Chlorella sp. and its acute toxicity to Daphnia, zebra fish and grass carps were observed, and its influence on main physicochemical factors of aquaculture water was also assayed. Results: The growth of Chlorella sp. was promoted with B. amyloliquefaciens microcapsules at the final concentrations of 0.2~2 000mg/L, its IC₅₀ to Chlorella sp. was 2 000mg/L, and its LD₅₀ to Daphnia, zebra fish and grass carps were all >2 000mg/L (or mg/kg body weight). In addition, in the period of 14 days after the input of the B. amyloliquefaciens microcapsule into the farming water at 0.2~2 000mg/L, the contents of the ammonia and sulfide as well as the pH values were gradually reduced, only the nitrite nitrogen concentration first rose slightly and followed by a slow decrease, and the changes of the physiochemical factors related negatively to the concentration of the B. amyloliquefaciens microcapsules. Conclusion: The B. amyloliquefaciens microcapsule was actually nontoxic, its effects on the ammonia, nitrite nitrogen, sulfide and pH in the fish faming water were under the control of their safe concentrations to aquatic animals, such as the goby fry, Pelteobagrus fulvidraco, Esox lucius, Procambarus clarkia. The present study provided important scientific basis on the safe use of B. amyloliquefaciens microcapsules in aquaculture.

Key words： Aquaculture Bacillus amyloliquefaciens Microcapsules Safety evaluation

Received: 08 December 2011 Published: 25 May 2012

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Cite this article:

CAO Hai-peng, WEI Ruo-peng, HE Shan, LU Li-qun. Safety Evaluation of Bacillus amyloliquefaciens Microcapsules Used for Aquaculture. China Biotechnology, 2012, 32(05): 58-65.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I05/58

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