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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (12): 63-71    DOI:
    
Cloning and Expression Patterns of PtFT1 and PtFT2 from Populus tomentosa
CHEN Zhong, LI Hao, LI Ying, WANG Jia, YE Mei-xia, GUO Bin, JI Le-xiang, AN Xin-min
National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing Forestry University, Beijing 100083, China
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Abstract  

Two FT-like genes named PtFT1 and PtFT2 were cloned by RT-PCR from Populus tomentosa. Sequencing results indicated that the length of both PtFT1 and PtFT2 is 525bp encoding 174 amino acids. Blast analysis showed that PtFT1 and PtFT2 respectively share over or more than 75% homology in amino acid sequence with Arabidopsis(Arabidopsis thaliana) FT(BAA77838.1), grape(Vitis vinifera) FT(ABI99465.1) and other FT homologues. The deduced amino acid sequence contained one conserved motifs and two critical residues. Phylogenetic tree analysis suggested both PtFT1 and PtFT2 were classified into the FT-clade. Furthermore, the expression patterns of PtFT1 and PtFT2 in different tissues were examined using real-time quantitative RT-PCR. It showed that the constitutive and stable transcripts of PtFT1 and PtFT2 were detected in each tissue, but the expression levels between PtFT1 and PtFT2 were different. The expressions of PtFT1 and PtFT2 in male and female floral bud at early stage on July 5th were apparently higher than at late stage on March 10th in the next year. In addition, the data indicated that the expression of PtFT1 and PtFT2 in Populus tomentosa responsed to daylength, PtFT1 and PtFT2 transcripts became abundant under long days. The results suggested that PtFT1 and PtFT2 might function in photoperiod pathway about regulating flowering in Populus tomentosa. This will contribute to understanding the molecular mechanism of PtFT1 and PtFT2 in light-dependent pathways and also will benefit to regulation of flowering.



Key wordsPopulus tomentosa      FT      Pattern of expression      qRT-PCR     
Received: 15 August 2011      Published: 25 December 2011
ZTFLH:  Q786  
Cite this article:

CHEN Zhong, LI Hao, LI Ying, WANG Jia, YE Mei-xia, GUO Bin, JI Le-xiang, AN Xin-min. Cloning and Expression Patterns of PtFT1 and PtFT2 from Populus tomentosa. China Biotechnology, 2011, 31(12): 63-71.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I12/63


[1] Amasino R M. Control of flowering time in plants. Curr Opin Genet Dev, 1996, 6(4): 480-487.

[2] Putterill J, Laurie R, Macknight R. It's time to flower: the genetic control of flowering time. Bioessays, 2004, 26(4): 363-373.

[3] Georges B, Claire P. A physiological overview of the genetics of flowering time control. Plant Biotechnol,2005, 3(1): 3-16.

[4] Maarten K, Carlos A B, Anton J M P, et al. Genetic control of flowering time in Arabidopsis. Annu Rev Plant Physiol Plant Mol Biol, 1998, 49: 345-370.

[5] Putterill J, Robson F, Lee K, et al. the constans gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 1995, 80(6):847-857.

[6] Park D H, Somers D E,Kim Y S, et al. Control of circadian rhythms and photoperiodic flowering by the Arabidopsis GIGANTEA gene. Science,1999, 285 (5433):1579-1582.

[7] Suarez-Lopez P, Wheatley K, Robson F, et al. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature, 2001, 410 (6832):1116-1120.

[8] Wigge P A, Kim M C, Jaeger K E, et al. Integration of spatial and temporal information during floral induction in Arabidopsis. Science, 2005, 309 (5737):1056-1059.

[9] Corbesier L, Vincent C, Jang S, et al. FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis. Science, 2007, 316(5827): 1030-1033.

[10] Mitsutomo A, Yasushi K, Sumiko Y, et al. FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science, 2005, 309(5737): 1052-1056.

[11] Tamaki S, Matsuo S, Wong H L, et al. Hd3a protein is a mobile flowering signal in rice. Science, 2007, 316(5827): 1033-1036.

[12] Kojima S, Takahashi Y, Kobayashi Y, et al. Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions. Plant Cell Physiology, 2002, 43(10): 1096-1105.

[13] Lifschitz E, Eshed Y. Universal florigenic signals triggered by FT homologues regulate growth and flowering cycles in perennial day-neutral tomato. Journal of Experimental Botany, 2006, 57(13): 3405-3414.

[14] Lifschitz E, Eviatar T, Rozman A, et al. The tomato FT ortholog triggers systemic signals that regulate growth and flowering and substitute for diverse environmental stimuli. Proc Natl Acad Sci USA, 2006, 103(16): 6398-6403.

[15] Bradley D, Ratcliffe O, Vincent C, et al. Inflorescence commitment and architecture in Arabidopsis. Science, 1997,275(5296):80-83.

[16] Kobayashi Y, Kaya H, Goto K, et al. A pair of related genes with antagonistic roles in mediating flowering signals. Science, 1999, 286(5446): 1960-1962.

[17] Hsu C Y, Liu Y X, Luthe D S, et al. Poplar FT2 shortens the juvenile phase and promotes seasonal flowering. Plant Cell, 2006, 18(8): 1846-1861.

[18] Bohlenius H, Huang T, Charbonnel-Campaa L, et al. CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees. Science, 2006, 312(5776): 1040-1043.

[19] Igasaki T, Watanabe Y, Nishiguchi M, et al. The FLOWERING LOCUS T/TERMINAL FLOWER 1 family in Lombardy poplar. Plant Cell Physiology, 2008, 49(3):291-300.

[20] Yuceer C, Land S B, Kubiske M E, et al. Shoot morphogenesis associated with flowering in Populus deltoides(Salicaceae). American Journal of Botany, 2003, 90:196-206.

[21] Chang S J, Jeff P, John C, et al. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biol Rep, 1993, 11(2): 113-116.

[22] Ahn J H, Miller D, Winter V J, et al. A divergent external loop confers antagonistic activity on floral regulators FT and TFL1. EMBO Journal, 2006, 25(3): 605-614.

[23] Endo T, Shimada T, Fujii H, et al. Ectopic expression of an FT homolog from citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.). Transgenic Research, 2005, 14(5): 703-712.

[24] Kotoda N, Hayashi H, Suzuki M, et al. Molecular characterization of flowering locus t-like genes of apple (malus×domestica borkh.). Plant Cell Physiol, 2010, 51(4):561-575.

[25] Mimida N, Goto K, Kobayashi Y, et al. Functional divergence of the TFL1 -like gene family in Arabidopsis revealed by characterization of a novel homologue. Genes to Cells, 2001, 6(4): 327-336.

[26] Yoo S Y, Kardailsky I, Lee J S, et al. Acceleration of flowering by overexpression of MFT (MOTHER OF FT AND TFL1). Mol Cells, 2004, 17(1): 95-101.

[27] Ayako Y, Yasushi K, Koji G, et al. TWIN SISTER OF FT (TSF) acts as a floral pathway integrator redundantly with FT. Plant Cell Physiol, 2005, 46(8): 1175-1189.

[28] Nishikawa F, Endo T, Shimada T, et al. Increased CiFT abundance in the stem correlates with floral induction by low temperature in Satsuma mandarin (Citrus unshiu Marc.). Journal of Experimental Botany, 2007, 58(14): 3915-3927.

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