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中国生物工程杂志

China Biotechnology
China Biotechnology  2012, Vol. 32 Issue (02): 69-75    DOI:
    
Construction of a DNA Library in Small Cassette Encoding Random Peptide Consisting of 15 Amino Acids
LU Ming-feng1,2, KANG Shou-kai3, ZHANG Yue-jie2, ZHANG Hong-yu3
1. School of Life Science, Shandong Normal University, Ji'nan 250014, China;
2. School of Life Science, Shandong University of Technology,Zibo 255091,China;
3. School of Life Science, Huazhong Agriculture University, Wuhan 430070, China
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Abstract  

Using a small cassette DNA encoding library construction strategies, DNA template encoding 10 random amino acids was synthesized according to degenerate codon of a 15 kinds amino acids set. After three consecutive small cassette library assembly process including PCR amplification, digestion and connection, a small cassette DNA encoding library which a random coding region with length up to 97 amino acids was successfully constructed, and library capacity was 1.31 × 1012/ml.



Key wordsRandom peptide library      Simplified amino acids alphabet      Library in small cassette     
Received: 01 December 2011      Published: 25 February 2012
ZTFLH:  Q10  
  Q-3  
Cite this article:

LU Ming-feng, KANG Shou-kai, ZHANG Yue-jie, ZHANG Hong-yu. Construction of a DNA Library in Small Cassette Encoding Random Peptide Consisting of 15 Amino Acids. China Biotechnology, 2012, 32(02): 69-75.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2012/V32/I02/69


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