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| Metabonomic Study on the Composition of Insect-resistant Transgenic Cottonseeds |
| SUN Cai-xia1, WANG Ying1, WU Xiao-fei1, CHEN Li-jun2, WU Zhi-jie2 |
1. Institute of Biotechnology, Northeastern University, Shenyang 110819, China;
2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China |
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Abstract The term of metabolome has been used to describe the responses of plant to external perturbations recently. The study of metabolite profiling of different insect-resistant cottonseeds and their non-transgenic counterparts using 1H NMR and multivariate analysis technique was performed to investigate the unintended metabolic variations associated with genetic modifications. The results obtained showed that the overall appearance of the spectrum was quite similar among different cottonseeds. However, there were many different peak signals in the animo acids region (3～0.5 ppm) of the expansion spectra of transgenic cottonseeds when compared with their controls. Although score plots generated using principal component analysis showed the potential to distinguish transgenic cottonseeds from non-transgenic controls, a better classification between them was obtained by partial least square-discriminant analysis. The major compounds contributing to the discrimination were those metabolites that involved the metabolic pathway of fatty acid, the primary nitrogen metabolism and the tricarboxylic acid cycle.
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Received: 21 June 2012
Published: 25 November 2012
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