|
|
|
| Effects of Temperature Stress on the Accumulating of Triterpenes and Defense Enzyme Activity in Suspension Cells of Birch (Betula platyphylla Suk.) |
| ZHAO Wei, YIN Jing, ZHAN Ya-guang, REN Chun-lin, WANG Yan, MA Hong-si, SU Xin |
| College of Life Sciences, Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Nation, Northeast Forestry University, Harbin 150040, China |
|
|
|
Abstract After 7-d-suspending-culture cells of Birch (Betula platyphylla Suk.) which were treated with two kinds of temperature stress: the high (50 ℃ for 1h) and low temperature (4℃ for 3h),respectively, they were continuously suspended culture for 0 h～7d, and during that time, the growth, accumulation of total triterpenes and oleanolic acid, and the activities of defense enzyme were investigated. The results showed that: for the total triterpenes, its accumulation significantly increased in the cells suspending-cultured for 24～3d after both the high and low temperature treatments; it was higher in these cells treated with high temperature than low temperature; and its highest was in the cells suspending for 48 h after high temperature treatment, higher 36.4% and 12.87% than those after control(25℃) and low temperature treatment, respectively. The accumulation of total triterpenes in the 4d～7d suspending-culture cells after low temperature treatment, also obviously increased, and higher 22.1% in the 7-d suspending-culture cells than that in control. For the oleanolic acid, its accumulation in the cells suspending-culutre for 15h～4 d was significantly improved after both the high and low temperature treatments, and they in the cells suspending-culture after high and low temperature treatments were higher 38.1% and 39.65% than control, respectively. The activities of defense enzyme: SOD, CAT, PAL and POD in the cells treated with the high and low temperature, were accelerated, and the accumulation of MDA (malondialdehyde) was higher. But their activities changes were different. Those results suggested that the interaction of these defense enzymes in cells relieved the free radical hurts, which made cells adapt to tremperature stress, and promote the secondary metabolism materials.
|
|
Received: 19 September 2012
Published: 25 February 2013
|
|
|
|
|
[1] Pisha E, Chai H, Lee I, et al. Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis. Nat Med, 1995, 1: 1046-1051.
[2] 李薇, 李岩, 金雄杰. 白桦三萜类物质的抗肿瘤作用及其对免疫功能的增强效应. 中国免疫学杂志, 2000, 16(9): 485-487. Li W, Li Y, Jin X J. Antitumor activity and immunoregulatory effect of triterpenes isolated from Betula platyphylla. Chinese Journal of Immunolugy, 2000, 16(09): 485-487.
[3] 李岩, 金雄杰, 谢湘林,等. 白桦三萜类物质抗黑色素瘤B16、S180肉瘤作用及其机制的实验研究. 中国药理学通报, 2000, 16(3): 279-281. Li Y, Jin X J, Xie X L. Antitumor activity and mechanism of tritrepenes isolated form Betula platyphylla on melanoma B16 and sarcoma 180. Chinese Pharmacological Bulletin, 2000, 16(3): 279-281.
[4] 汤华成,张东杰,赵蕾.功能性食品因子齐墩果酸研究进展.食品科技,2007(9):16-18. Tang H C, Zhang D J, Zhao L, Advances in the research of oleanolic acid, advances in the research of oleanolic acid. Food Science and Technology, 2007(9): 16-18.
[5] 胡芳,赵智慧,刘孟军.HPLC法测定不同枣品种果实中白桦酯酸、齐墩果酸和熊果酸含量.中国农学通报,2011,27(05):434-438. Hu F, Zhao Z H, Liu M J. The determination of betulic acid, oleanic acid and ursolic acid in different Chinese jujube cultivars by HPLC. Chinese Agricultural Science Bulletin, 2011, 27(05): 434-438.
[6] 鲁守平,隋新霞,孙群,等.药用植物次生代谢的生物学作用及生态环境因子的影响.天然产物研究与开发,2006,18:1027-1032. Lu S P, Sui X X, Sun Q. Biological functions of secondary metabolism of medicinal plants and influences of ecological environment. Natural Product Research and Development, 2006, 18: 1027-1032.
[7] 黄璐琦,陈美兰,肖培根.中药材道地性研究的现代生物学基础及模式假说.中国中药杂志,2004,29:494-496. Huang L Q, Chen M L, Xiao P G. The research modernbiological basis and mode hypothesis of traditional Chinese medicinal materials. China Journal of Chinese Materia Medica, 2004,29(6): 494-496.
[8] 徐茂军, 董菊芳, 张新波. NO 和H2O2 在介导热激诱发金丝桃细胞合成金丝桃素中的信号互作.中国科学 C 辑:生命科学 2008 ,38 (7): 643-653. Xu M J, Dong J F, Zhang X B. NO and H2O2 signal interaction in medium heat induced hypericin synthetic of hypericum cell. Science in China C: Life Science, 2008, 38 (7): 643-653.
[9] Prasad T K, Anderson M P , Martin B A. Evidence for chilling induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide.Plant Cell , 1994 , 6 : 65-74.
[10] Pecetti L, Tava A, Romani M, et al. Variety and environment effects on the dynamics of saponins in Lucerne (Medicago sativa L.). Eur J Agron, 2006, 25(3): 187-192.
[11] 郝征, 张钢, 李亚.不同高温胁迫对白桦幼苗几个生理生化指标和电阻抗图谱参数的影响.西北植物学报, 2010, 30( 9) : 1844- 1851. Hao Z, Zheng G, Li Y. Physiological and biochemical indexes and electrical Impedance spectroscopy of Betula platyphylla seedlings under heat stress. Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(9): 1844-1851.
[12] 陈莎莎, 刘鸿雁,郭大立. 内蒙古东部天然白桦林的凋落物性质和储量及其随温度和降水梯度的变化格局.植物生态学报,2010,9:111-116. Chen S S, Liu H Y, Guo D L. Litter stocks and chemical quality of natural birch forests along temperature and precipitation gradients in eastern Inner Mongolia. Chinese Journal of Plant Ecology, 2010, 9: 111-116.
[13] 王博 范桂枝 詹亚光.不同培养基类型和植物生长调节剂配比对白桦愈伤组织中三萜积累的影响.林业科学,2008,10(6):153-158. Wang B, Fan G Z. Zhan Y G. Effects of different medium types and plant growth regulator combinations on accumulation of triterpenoids in birch (Betula platyphylla) calli. Scientia Silvae Sinicae, 2008, 10(6): 153-158.
[14] 谭朝阳,袁宏佳.HPLC 法测定龙芽楤木药材中齐墩果酸的含量.中国中医药信息杂志,2010,1(17):46-47. Tan Z Y, Yuan H J. Determination of oleanolic acid in cortex Aralia elatae by HPLC. Chinese Journal of Information on Traditional Chinese Medicine, 2010, 1(17): 46-47.
[15] Yin J, Zhan Y G, Ren C L. Distribution and expression characteristics of triterpenoid and OSC genes in white birch (Betula platyphylla suk.). Molecular Biology Reports, 2012,2:1117-1121.
[16] 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.164-165. Li H S. Principle and Technology of Experiment of Plant Physiological and Biochemical. Beijing: Higher Education Press, 2000.164-165.
[17] 冷平山,张国军,吴晓云,等.秋冬季节柿属植物树体内酚类物质含量的变化.中国农业大学学报,2001,5:56-61. Leng P S, Zhang G J, Wu X Y. Change of phenols in persimmon during autumn and winter. Journal of China Agricultural University, 2001, 5: 56-61.
[18] Du X M. The production and scavenging of reactive oxygen species in plants.Chin J Biotec,2001,17(2):121-126.
[19] 张成军,郭佳秋,陈国祥,等.高温和干旱对银杏光合作用、叶片中黄酮苷和萜类内酯含量的影响.农村生态环境,2005, 21(3):11-15. Zhang C J, Guo J Q, Chen G X. Effects of high temperature and/or drought on growth and secondary metabolites in Ginkgo biloba leaves. Rural Eco-environment, 2005, 21(3): 11-15.
[20] Kocsy G, Gabriella S, Jozsef S, et al. Heat tolerance together with heat stress-induced changes in glutathione and hydroxymethy glutathione levels is affected by chromosome 5A of wheat.Plant Sci,2004, 166:451-458.
[21] Sun W, Montagu M W, Verbruggen N. Small heat shock proteins and stress tolerance in plants. Biochem Biophys Acta, 2002, 1577:1-9.
[22] Yan S, Zhang Q, Tang Z, et al. Comparative proteomic analysis provides new insights into chilling stress responses in rice. Mol Cell Proteomics, 2006, 5:484-496.
[23] Yeh D M, Lin H F. Thermostability of cell membranes as a measure of heat tolerance and relationship to flowering delay in chrysanthemum.American Society for Horticultural Science, 2003, 128: 656-660.
[24] Howarth C J. Genetic improvements of tolerance to high temperature.In: Ashraf M, Harris P J. Abiotic Stress:Plant Resistance Through Breeding and Molecular Approaches.New York: Howarth Press Inc.,2005.
[25] Larkindale J, Knight M R. Protection against heat stress-induced oxidation damage in Arobidopsis involves calcium, abscisic acid, ethylene,and salicylic acid. Plant Physiology, 2002,128: 682-695.
[26] 孙学成,胡承孝,魏文学.钼肥对冬小麦脯氨酸和抗坏血酸含量的影响.三峡大学学报,2001,23(5):473-476. Sun X C, Hu C X, Wei W X. Effects of Mo fertilizer on proline content and ascorbic acid content in winter wheat. Journal of University of Hydraulic and Electric Engineering/Yichang, 2001, 23(5): 473-476.
[27] 吴建慧,杨玲,孙国荣.低温胁迫下玉米幼苗叶片活性氧的产生及保护酶活性的变化.植物研究,2004,4:115-120. Wu J H, Yang L, Sun G R. Generation of activated oxygen and change of cell defense enzyme activity in leaves of maize seedling under the stress of low temperature. Bulletin of Botanical Research, 2004, 4: 115-120. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
