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| The C-terminal Residues of the Cucumber Mosaic Virus 2b Protein are Not Responsible for RNA Silencing Suppressor Activity |
| WANG Sheng1,2, CAO Min1, DONG Jie1, MU Hong-zhen1, DING Guo-ping1, ZHANG Hong1 |
1. School of Life Science, Ning Xia University, Yinchuan 750021, China;
2. Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China, Yinchuan 750021, China |
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Abstract To gain insights into the function of the conserved residues in the C-terminal of the Cucumber mosaic virus (CMV) -encoded silencing suppressor protein 2b, the 2b protein of Q-CMV, a subgroup II isolate, and 2bdelC, a C terminal truncated mutant of Q 2b, were individually constructed into plant expression vectors. Those two vectors were co-delivered into Tobacco plants(Nicotiana benthamiana)with a Potato virus X-derived expression vector (PVXdt-GFP) by means of an agrobacterium-mediated transient transformation assay and then analyzed for their silencing suppressor activities. Western blotting was performed to monitor the protein level of 2b and its mutants. The results showed that the truncation did not alter the accumulation of Q 2b protein in plant cell and this indicated that the C-terminal residues are not crucial for the stability of 2b protein in vivo. We further show that the co-expressed GFP have indistinguishable expression levels in the presence of Q 2b protein or the Q 2b C-terminal mutant. The results implicate the C-terminal residues neither have effect on the suppressor activity nor contain any small RNA binding domains.
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Received: 24 September 2012
Published: 25 January 2013
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