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| Expression of Enterovirus 71 VLP in Baculovirus System and Evaluation of Its Immunogenicity |
| GUO Li-li, OU Xia, MI Kai, SUN Mao-sheng, LI Hong-jun |
| Yunnan Engineering Center of Vaccine Research Development on Severe Infecticus Diseases Yunnan Keylaboratory of Vaccine Research & Development on Severe Infections Diseases, Kunming 650118, China |
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Abstract Enterovirus type (EV71) is an etiologic agent responsible for seasonal epidemics of hand-foot-and-mouth disease and causes outbreaks with significant mortality among young children. Expression of enterovirus EV71 Virus-like Particles(VLP) in Bac-to-Bac Baculovirus Expression System and evaluated its immunogenicity. Recombinant AcMNPV-P1-3CD was obtained by transfecting the Bacmid-P1-3CD into the insect cell line of Sf9. The expressed protein was analyzed by IFA, Western blot methods and electron microscope obseruation, The VLPs of EV71 were observed by Electron microscope, while its immunogenicity was measured by ELISA and Microneutralization. The result reveals that EV71 VLP expressed by Recombinant AcMNPV-P1-3CD has strong immunogenicity, which is helpful for the development of the vaccine against EV71 infection.
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Received: 25 October 2012
Published: 25 January 2013
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