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Abstract The SRY gene from Buffalo (Bubalus bubalis) genome was amplified by the polymerase chain reaction (PCR) with primers based on the sequence of Hostein SRY gene. The amplified fragment was 2005 bp include 5’ UTR(1~504bp) and 3’UTR(1196-2005bp). And The amplified fragment was cloned and sequenced. Sequence analysis showed that the coding region of SRY gene(505~1195bp) from Buffalo was highly homologous with those of other Bovine counterpart genes (96% homology), especially in the HMG box region (99%homology). It was found that there were only signal on male Buffalo genome on Southern blot,which indicate SRY gene are highly conservative on evolves.
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Received: 23 February 2006
Published: 25 July 2006
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