Abstract As a prefatory topic in genetic engineering technology, the construction of transgenic bacteria has the good industry prospect. Construction includes its best expression vector construction, the choice of suitable host cells, transforming, the identification and screening of genetic stability, the genetic stability of the plasmid, and high-level expression of the transgenic bacteria. This article reviews the transgenic engineering bacteria and vector about the construction, the acceptor bacteria such as Escherichia coli, lactic acid bacteria, yeasts and cyanobacteria selection and breeding, exogenous gene expression and application in aspect on agriculture of genetic engineering feed phytase bacteria, pesticides and pesticide Rhizobium microbial genetic engineering, and pointed out the safety problem and the imperfect aspect of transgenic bacteria in the application.
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