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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (02): 94-99    DOI: Q819
    
Optimization of Plasmid Extraction Method from Lactic Acid Bacteria
1.College of Bioengineering,Henan University of Technology,Zhengzhou 450001,China
2.Zhongyuan University of Technology,Zhengzhou 450007,China
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Abstract  

By combining the extraction method of plasmid from E.coil and the method of plasmid from lactic acid bacteria reported before to improve the common methods used in plasmid extraction kit,a fast,safe and high efficient method for extraction of plasmid DNA from lactic acid bacteria was developed.The optimal concentration of lysozyme was proved to be 20mg/ml,the optimal processing time was 30min,and the usage toxic ethidium bromide was avoided during the whole process of extraction. Experimental results showed that,the developed method showed an increased extraction efficiency,as well as good reproducibility,and thus provided a good foundation for the following molecular improvement of lactic acid bacteria.



Key wordsIactic acid bacteria      Extraction of plasmid      Method optimization     
Received: 13 November 2009      Published: 26 February 2010
Cite this article:

MO Qian, CAO Jian, TUN Xin-Quan, LI Yan-Fang, LI Feng-Juan, SONG Feng. Optimization of Plasmid Extraction Method from Lactic Acid Bacteria. China Biotechnology, 2010, 30(02): 94-99.

URL:

https://manu60.magtech.com.cn/biotech/Q819     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I02/94

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