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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (04): 113-118    DOI:
    
An Efficient Method for Adventitious Shoot Regeneration from Leaf and Stem-segment Explants of Russian Olive Elaeagnus angustifolia L.
LU Song-chong1,2, ZHU Jin-qi2, LI Jie1, ZHANG Hong-xia2
1. College of Life Science, Northeast Agriculture University, Harbin 150030,China;
2 National Laboratory of Plant Molecular Genetics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
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Abstract  

The establishment of an efficient adventitious shoot regeneration system from leaf discs and stem segments of one-year-old Russian olive (Elaeagnus angustifolia L.)was established. Shoot regeneration using leaf explants and stem segments was obtained on MS medium supplemented with different cytokinins viz., 6-benzyladenine (BA) and Zeatin (Zt), with combination of auxins viz., naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA). MS + 0.5mg/L 6-BA + 0.2mg/L NAA was found to be a better combination for leaf regeneration, producing a maximum of 4.3 shoots per explant, whereas MS + 1.0mg/L Zt+0.5mg/L NAA for stemsegment explants produced the highest number of multiple shoots (3.6) per explant. The regenerated shoots produced 100% rooting on 1/2 strength MS medium supplied with 0.5mg/L NAA. In vitro raised plantlets were successfully transferred to pots containing sterile soil∶peat moss∶sand (1∶1∶1) for acclimatization, and 77% of rooted plantlets survived under ex situ conditions. The currently employed regeneration protocol through leaf explants and stem segments facilitates germplasm conservation of this medicinally and ecologically important species besides providing a standardized regeneration protocol for genetic transformation studies in the future.



Key wordsRussian olive      Leaf explants      Shoot segment      in vitro regeneration      Multiple shoots     
Received: 20 December 2010      Published: 26 April 2011
ZTFLH:  Q819  
Cite this article:

LU Song-chong, ZHU Jin-qi, LI Jie, ZHANG Hong-xia. An Efficient Method for Adventitious Shoot Regeneration from Leaf and Stem-segment Explants of Russian Olive Elaeagnus angustifolia L.. China Biotechnology, 2011, 31(04): 113-118.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I04/113


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