|
|
Abstract Objective: To study expression of the gene of n-6 fatty acid desaturase fat-1 in human breast cancer cell, composition change of fatty acids of cell membrane, and effect of the gene on apoptosis of breast cancer cell. Methods: Construct recombinant adenovirus vector (Ad.GFP.fat-1) containing fat-1 gene, the recombinant adenovirus was produced in 293 package cell, then it infected the breast cancer cells MCF-7; total RNA of the cells was isolated and hybridized with antisense RNA probe of fat-1 mRNA by Northern to analyze the expression of fat-1 in MCF-7; the effect of fat-1on the proliferation of MCF-7 cell was analyzed by MTT method，apoptosis of the cells were detected by apoptosis kit; content of n-6 PUFAs/n-3 PUFAs was analyzed by Gas Chromatography. Results: The proposed recombinant virus was got through DNA recombinant technique; fat-1 gene effectively expressed in human breast cancer cell MCF-7; the fat-1 mRNA band appeared 2 days after infection of virus Ad.GFP.fat-1; compared with the control cell (Ad.GFP), proliferation of MCF-7 cell was markedly inhibited by the gene fat-1( 23%, p<0.05) and apoptosis of the cells was promoted; moreover, fat-1 gene decreased ratio of n-6 PUFAs/n-3 PUFAs of MCF-7 cell membrane. Conclusion: The fat-1gene transfer mediated by adenovirus could heterologously express in human breast cancer cell MCF-7, inhibit proliferation of the cell. The mechanism analysis shown that fat-1 decreased ration of n-6 PUFAs/n-3 PUFAs of cell membrane.
|
|
Received: 22 September 2005
Published: 25 May 2006
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
