中国生物工程杂志

Gene trap vector pU17 can be integrated into chromosome DNA of human hepatoma cells randomly. After selected with G418, X-gal staining and PCR checking, several high X-gal expression cell colonies were obtained. A special Hepatitis B virus (HBV) polymerase full-length cDNA vector containing mutant loxP sites exchanged -geo DNA fragment of gene trap vector in these colonies with the Cre enzyme. Under rigorously selection of puromycin, a new cell line expressing HBp-His fusion protein was established. HBp protein could be identified well with the His-tag antibody. This hepatoma cell line might be a useful tool for preparation HBp protein antigen and analysis of its function.