中国生物工程杂志

High-voltage electrostatic system was used to prepare alginate-chitosan microcapsules for Immunoglobulin of Yolk (IgY) via electrostatics interaction. Optimum conditions were established for preparation of homogenous, spherical, and smooth alginate-chitosan microcapsules. The entrapment efficiency , in vitro release of the microcapsules in SGF (Simulated Gastric Fluid) and SIF (Simulated Intestinal Fluid) and IgY activity retained were examined. IgY entrapment efficiency achieved 90% in the optimum condition. The IgY retained 80% activity after 2 h exposure to SGF and then 6 h exposure to SIF. The microcapsules released less than 10% IgY upon exposure to SGF for 2 hours, and released more than 90% IgY upon exposure to SIF for 6 hours. Electrophoretic studies of IgY microcapsules passed gastrointestinal tract showed the intact protein band. Consequently, it can be said that the system can be offered for the temporary protection of IgY against acidic and enzymatic degradation during gastric passage, enabling its use for oral passive immunotherapy.