The study of CB-MSCs differentiating to become dopaminergic neuron-like cells

China Biotechnology

2009, Vol. 29

Issue (03): 1-8 DOI:

The study of CB-MSCs differentiating to become dopaminergic neuron-like cells

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Abstract

Abstract : Objective :To detect the dopaminergic neural cells differentiated pathway, the related gene in dopaminergic neural cells development process were investigated. The role of Human amnion epithelial conditional media (ACM) and SHH, FGF8 was focused on in cultivation process of human umbilical cord blood mesenchymal stem cells (CB-MSCs). Method: Preparation of the mononuclear cell fraction was performed by the Ficoll gradient technique according to the manufacturer’s instructions . ACM and SHH, FGF8 were combined to induce CB-MSCs, and there were four groups CON, ACM, SHH+FGF8, ACM+SHH+FGF8. After 48 hours of co-cultivation , Immunophenotypes of CB-MSCs were observed by immunocytochemistry and the related gene in dopaminergic neural cells development process En-1、Foxa2、Lmx1b、Gli-1、Pitx3，Nurr1 and Ngn2 were detected by RT-PCR. Results: After 48 hours of co-cultivation ，the positive cells numer of TH and DAT were higher in induction groups than in control group. By RT-PCR, En-1，Foxa2，Pitx3, Lmx1b were higher in ACM, SHH+FGF8, ACM+SHH+FGF8 groups than in the control group(p<0.01). Nurr1,Ngn2 were strongly higher in SHH+FGF8. Conclusion:The related gene in dopaminergic neural cells development process，En-1、Foxa2、Lmx1b、Gli-1、Pitx3，Nurr1 and Ngn2 play a role in the differentiated pathway form CB-MSCs to dopaminergic neural cells.

Key words： CB-MSCs;dopaminergic neuron-like cells;ACM;SHH+FGF8;induction

Received: 30 October 2008 Published: 31 March 2009

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	LIN Yan- Sun-Hai-Mei- Yang-Hui- Ceng-Xiao-Bei- Ji-Feng-Qing

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LIN Yan- Sun-Hai-Mei- Yang-Hui- Ceng-Xiao-Bei- Ji-Feng-Qing. The study of CB-MSCs differentiating to become dopaminergic neuron-like cells. China Biotechnology, 2009, 29(03): 1-8.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I03/1

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