Batch Culture of Magnetic Acidthiobacillus ferrooxidans by Fed-batch Fermentation

doi:Q93-335

China Biotechnology

2010, Vol. 30

Issue (06): 109-112 DOI: Q93-335

Batch Culture of Magnetic Acidthiobacillus ferrooxidans by Fed-batch Fermentation

LIU Xin-xing,GUO Ning,LIANG Wan-jie,ZHANG Jian

School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China

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Abstract

Acidithiobacillus ferrooxidans (A.f) could synthesize intra-cellular nanometer magnetic particles. With the development of study on their culture conditions, the research of magnetotaxis bacteria will get more benefit from it. By fed-batch fermentation magnetic A.f could be batch cultured in the laboratory. In the shake-flask fermentation, the final volume of liquid was 2 L. After 40 h in the fermentation Fe2+ (5%) was added in the fermentation liquid. The max cell density could reach 2.33×107cells·ml-1. Compared with control experiments, it increased by 60.69%. At the same condition, the completion 9K culture medium was added in the fermentation liquid. The max cell density could reach 2.47×107cells·ml-1. Compared with control experiments, it increased by 70.34%. And atom force microscopy and magnetic force microscopy showed that cells in the fermentation were magnetic.

Key words： Magnetic Acidithiobacillus ferrooxidans Fed-batch fermentation Atom force microscopy

Received: 01 February 2010 Published: 12 June 2010

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	LIU Xin-Xing
	GUO Ning
	LIANG Mo-Ji
	ZHANG Jian

Cite this article:

LIU Xin-Xing, GUO Ning, LIANG Mo-Ji, ZHANG Jian. Batch Culture of Magnetic Acidthiobacillus ferrooxidans by Fed-batch Fermentation. China Biotechnology, 2010, 30(06): 109-112.

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https://manu60.magtech.com.cn/biotech/Q93-335 OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I06/109

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