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中国生物工程杂志

China Biotechnology
China Biotechnology  2017, Vol. 37 Issue (9): 65-70    DOI: 10.13523/j.cb.20170909
    
Elevated Temperature Induced Acceleration of Electrophoretic Tissue Clearing Process of Rat Liver Tissue
YUAN Xiao-chuan1, LIAN Fang2, ZHAO Yin-nong1, CHEN Chuang1, HUANG Shan1, LI Ke-zhi1, ZENG Ai-ping1, HE Jian-bo1, WU Guo-bin1
1. Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China;
2. Department of Physiology, Basic Medical School of Guangxi Medical University, Nanning 530021, China
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Abstract  Objective:CLARIT is a morphological technique that produces structurally intact, yet optically transparent tissue, which has important applications in nervous system structure and function investigations. However in other organs, especially liver tissue, the researches are not comprehensive. Since the lipid in liver tissue is difficult to clear. The protocol is aim to make this process faster and easier. Method:The rat livers are fixed with hydrogel and cut into 1mm slices. The control group is cleared with passive method. On the other side, the experimental group is dealt with electrophoretic tissue clearing and elevate temperature as well to find the optimum temperature for the liver tissue clearing. Results:Under the condition of 12V 37℃,the experimental group become transparent after 48h electrophoresis, the relative-transparency is changeless when run with another 48h electrophoresis. If elevate the temperature of electrophoresis, after 48h basal electrophoresis (12V 37℃), the relative -transparency can be further improved. Conclusion:To create extremely clear liver tissue,the best electrophoretic tissue clearing procedure is to run the samples at 12V 37℃ for 48h followed by more 48h with the temperature increased to 52℃.

Key wordsRelative-transparency      Electrophoretic temperature      Lipid clearing      Liver slices      CLARITY     
Received: 22 May 2017      Published: 25 September 2017
ZTFLH:  Q819  
Cite this article:

YUAN Xiao-chuan, LIAN Fang, ZHAO Yin-nong, CHEN Chuang, HUANG Shan, LI Ke-zhi, ZENG Ai-ping, HE Jian-bo, WU Guo-bin. Elevated Temperature Induced Acceleration of Electrophoretic Tissue Clearing Process of Rat Liver Tissue. China Biotechnology, 2017, 37(9): 65-70.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170909     OR     https://manu60.magtech.com.cn/biotech/Y2017/V37/I9/65

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