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中国生物工程杂志

China Biotechnology
China Biotechnology  2017, Vol. 37 Issue (7): 12-17    DOI: 10.13523/j.cb.20170705
    
The Effect of PHP14 Knockdown on Lung Cancer Cells Apoptosis and Its Mechanism
XU An-jian1, LI Yan-meng1, LI Si-wen1, WU Shan-na2, ZHANG Bei1, HUANG Jian1
1. Experimental Center, Beijing Friendship Hospital, Beijing 100875, China;
2. Clinical Laboratory Center, Beijing Friendship Hospital, Beijing 100875, China
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Abstract  Objective: To knockdown PHP14 expression in lung cancer cells, and investigate the effect of PHP14 knockdownon lung cancer cells apoptosis and its mechanism. Methods: Using shRNA stably knockdown PHP14 expression in lung cancer cell A549; detected apoptosis of control cells and PHP14 RNAi cells under normal culture and apoptosis-induced treatment using double staining of Annexin V and PI by flow cytometry; investigated the tumor growth of control cells and PHP14 RNAi cells in vivo, and detected the expression of apoptosis related genes by Realtime-PCR and Western blotting. Results: The expression of PHP14 was stably knockdown successfully, and knockdown of PHP14 could promote induced-apoptosis, as well as the tumor growth in vivo; furthermore, the effect of PHP14 knockdown on lung cancer cells apoptosis possobly via Bcl-2 suppression was found. Conclusion: knockdown of PHP14 could promote induced-apoptosis possobly via Bcl-2 suppression.

Key wordsTumor growth in vivo      Bcl-2      Apoptosis      PHPT1     
Received: 05 March 2017      Published: 25 July 2017
ZTFLH:  Q819  
Cite this article:

XU An-jian, LI Yan-meng, LI Si-wen, WU Shan-na, ZHANG Bei, HUANG Jian. The Effect of PHP14 Knockdown on Lung Cancer Cells Apoptosis and Its Mechanism. China Biotechnology, 2017, 37(7): 12-17.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170705     OR     https://manu60.magtech.com.cn/biotech/Y2017/V37/I7/12

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