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The Effect of PHP14 Knockdown on Lung Cancer Cells Apoptosis and Its Mechanism |
XU An-jian1, LI Yan-meng1, LI Si-wen1, WU Shan-na2, ZHANG Bei1, HUANG Jian1 |
1. Experimental Center, Beijing Friendship Hospital, Beijing 100875, China; 2. Clinical Laboratory Center, Beijing Friendship Hospital, Beijing 100875, China |
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Abstract Objective: To knockdown PHP14 expression in lung cancer cells, and investigate the effect of PHP14 knockdownon lung cancer cells apoptosis and its mechanism. Methods: Using shRNA stably knockdown PHP14 expression in lung cancer cell A549; detected apoptosis of control cells and PHP14 RNAi cells under normal culture and apoptosis-induced treatment using double staining of Annexin V and PI by flow cytometry; investigated the tumor growth of control cells and PHP14 RNAi cells in vivo, and detected the expression of apoptosis related genes by Realtime-PCR and Western blotting. Results: The expression of PHP14 was stably knockdown successfully, and knockdown of PHP14 could promote induced-apoptosis, as well as the tumor growth in vivo; furthermore, the effect of PHP14 knockdown on lung cancer cells apoptosis possobly via Bcl-2 suppression was found. Conclusion: knockdown of PHP14 could promote induced-apoptosis possobly via Bcl-2 suppression.
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Received: 05 March 2017
Published: 25 July 2017
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