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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2020, Vol. 40 Issue (6): 74-83    DOI: 10.13523/j.cb.2001020
综述     
群体感应在动态代谢调控中的研究进展 *
薛艳婷,吴胜波,徐程杨,袁博鑫,杨书鹃,刘家亨,乔建军,朱宏吉()
天津大学化工学院 系统生物工程教育部重点实验室 天津化学化工协同创新中心合成生物学平台 天津 300072
Research Progress on the Quorum Sensing in the Dynamic Metabolic Regulation
XUE Yan-ting,WU Sheng-bo,XU Cheng-yang,YUAN Bo-xin,YANG Shu-juan,LIU Jia-heng,QIAO Jian-jun,ZHU Hong-ji()
Key Laboratory of the Ministry of Education on Bio-engineering System, School of Chernical Engineering and Technology, Tianjin University, Synthetic Biology Platform of Tianjin Chemical Industry Collaborative Innovation Center, Tianjin 300072, China
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摘要：

群体感应(quorum sensing,QS)是一种特殊的动态代谢调控机制,是细菌用于监控自身群体密度的环境信号感受系统。近年来,随着合成生物学的大力发展,基于稳定的菌群关系的人工合成菌群以及混菌共培养技术也取得了突破性的进展。群体感应系统因为可以实现细菌自主控制菌群关系的目的,而在菌群关系构建以及代谢工程中的研究和应用受到越来越多的关注。在对群体感应系统进行概述的基础上,对单菌基于群体感应的动态代谢调控进行了总结;同时也对群体感应的动态调控在革兰氏阴性菌和革兰氏阳性菌之间以及混菌共培养过程中的研究进展进行综述,以期能对群体感应系统的其他应用提供一些建议和帮助。
关键词： 代谢工程;  动态代谢调控;  群体感应;  菌群关系;  合成菌群    
Abstract:

Quorum sensing is a special dynamic metabolic regulation mechanism. It is an environmental signal sensing system used by bacteria to monitor their own population densities. In recent years, with the vigorous development of synthetic biology, breakthroughs have also been made in the artificially synthesized flora and mixed bacteria co-culture technology based on stable flora relations. The quorum sensing system can achieve the purpose of autonomously controlling the relationship between bacteria and bacteria, and its research and application in metabolic engineering has received more and more attention. Based on the overview of quorum sensing, this paper summarizes the dynamic metabolic regulation of single bacteria based on quorum sensing; at the same time, research progress in the dynamic regulation of quorum sensing between Gram-negative and Gram-positive bacteria and co-cultivation is reviewed. In order to provide some suggestions and help for other quorum sensing applications.
Key words: Metabolic engineering    Dynamic metabolic regulation    Quorum sensing    Flora relationship    Synthetic microbial consortia
收稿日期: 2020-01-06 出版日期: 2020-06-23
ZTFLH:  Q819  
基金资助: * 国家重点研发计划(2017YFD0201400)
通讯作者: 朱宏吉     E-mail: zhj@tju.edu.cn
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引用本文:

薛艳婷,吴胜波,徐程杨,袁博鑫,杨书鹃,刘家亨,乔建军,朱宏吉. 群体感应在动态代谢调控中的研究进展 *[J]. 中国生物工程杂志, 2020, 40(6): 74-83.

XUE Yan-ting,WU Sheng-bo,XU Cheng-yang,YUAN Bo-xin,YANG Shu-juan,LIU Jia-heng,QIAO Jian-jun,ZHU Hong-ji. Research Progress on the Quorum Sensing in the Dynamic Metabolic Regulation. China Biotechnology, 2020, 40(6): 74-83.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2001020    或    https://manu60.magtech.com.cn/biotech/CN/Y2020/V40/I6/74

分类 信号分子简称 表达蛋白 调节蛋白 产生菌株 文献
革兰氏阴性菌 3-oxo-C6-HSL LuxI LuxR 费氏弧菌V. fischeri [30]
C4-HSL RhlI RhlR 铜绿假单胞杆菌(Pseudomonas aeruginosa) [31]
3-O-C12-HSL LasI LasR 铜绿假单胞杆菌P. aeruginosa [31]
IV-HSL BjaI BjaR 缓生根瘤菌(Bradyrhizobium japonicum) [32]
3OH-C4-HSL LuxL/LuxM LuxN 哈维弧菌(Vibrio harveyi) [33]
p C-HSL RpaI RpaR 沼泽红假单胞菌(Rhodopseudomonas palustris) [34]
cinnamoyl-HSL BraI BraR 缓生根瘤菌 BTAi(Bradyrhizobium BTAi1) [35]
3-OH PAME PhcB PhcS 青枯雷尔氏菌(Ralstonia solanacearum) [36]
CAI-1 CqsA CqsS 霍乱弧菌(Vibrio cholerae) [37]
DSF RpfF RpfC 野油菜黄单胞菌(Xanthomonas campestris) [38]
革兰氏阳性菌 AgrD AgrB/D AgrC/A 金黄色葡萄球菌S. aureus [39]
Nisin NisA/B/C/P/T NisR/ K 乳酸如球菌L. lactis [40]
GBAP FsrD FsrA/C 肠球菌(Enterococcus faecalis) [41]
ComS ComA/B/C ComD/E 嗜热链球菌(Streptococcus thermophilus) [42]
subtilin SpaC/T/B SpaR/K 枯草芽孢杆菌(Bacillus subtilis) [43]
菌间信号 AI-2 LuxS/LsrB LuxP 多种 [25]
indole TnaA PykA/ DksA 多种 [28,44]
表1  常见的QS系统及其来源
图1  阴性菌的基于QS的动态代谢调控机制
图2  阴性菌间基于QS系统的菌群调控
图3  阳性菌的基于QS的菌群调控
图4  混菌体系QS系统的代谢调控
QS 机制 QS 来源 QS 应用菌种 代谢产物 成果 文献
lux V. fischeri E. coli 丝氨酸 最大理论生产率提高29.6% [46]
lux V. fischeri E. coli BW25113 异丙醇 产量与转化率分别提高了3倍和2.3倍 [47]
lux V. fischeri E. coli W3110 1,4-丁二醇 产量达到0.44g/L [48]
esa P. stewartii E. coli MG1655 肌醇
葡糖二酸
莽草酸		 肌醇产量提高5倍;葡糖二酸产量由0提高到>0.8 g/L;莽草酸产量从0提高到>100 mg/L [49]
lux
esa		 V. fischeri
P. stewartii		 BL21(DE3) 柚皮素
水杨酸		 柚皮素产量提升到463±1μM,水杨酸产量提高了1.8倍达到520±7 mg/L [50]
lux V. fischeri G. oxydans H24 维生素C 一步发酵稳定生产维生素C,2-KAG产量达到68.80±4.18 g/L [63]
lux V. fischeri E. coli BW25113 异丙醇 使用纤维二糖直接生产异丙醇 [64]
表2  基于QS的动态代谢调控策略在代谢工程中的应用一览表
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