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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2019, Vol. 39 Issue (4): 106-113    DOI: 10.13523/j.cb.20190414
综述     
细菌中D-氨基酸生物合成及调控作用研究进展 *
薛二淑1,2,3,吴昊1,2,3,宋倩倩1,2,3,田开仁1,2,3,乔建军1,2,3,财音青格乐1,2,3**()
1 天津大学化工学院 天津 300072
2 系统生物工程教育部重点实验室 天津 300072
3 天津化学化工协同创新中心合成生物学平台 天津 300072
Research Progress in the Biosynthesis and Regulation of D-amino Acids in Bacterial
Er-shu XUE1,2,3,Qian-qian SONG1,2,3,Kai-ren TIAN1,2,3,Jian-jun QIAO1,2,3,Cai-yin QINGGELE1,2,3**()
1 School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
2 Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China
3 Syn Bio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China
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摘要：

细菌中普遍存在L/D型氨基酸,与L-氨基酸(L-AAs)不同,D-氨基酸(D-AAs)不参与蛋白质合成,而与细胞壁肽聚糖的合成有关,直接影响细菌细胞壁的形状、数量和强度。D-AAs在细菌表征、药物抑菌性、靶标确定等方面具有重要的作用。目前,外源添加D-AAs参与肽聚糖合成的机制已有一些研究进展,其荧光衍生物已应用于细菌可视化,特异性探测细胞壁形成/重塑、细菌生长和细胞形态。但D-AAs如何影响细菌生长及其抗逆性的机制尚未研究清楚。对D-AAs的研究现状进行综述,重点介绍D-AAs在细菌中的生物合成机制和参与细胞壁合成的机制、非典型D-AAs对细菌的调控以及在细菌可视化中的应用,并对D-AAs未来研究方向进行了展望。
关键词： 肽聚糖;  D-氨基酸;  氨基酸异构酶;  氨基酸转氨酶    
Abstract:

L/D amino acids are common components in bacteria. Unlike L-amino acids (L-AAs), D-amino acids (D-AAs) are not involved in protein synthesis, but participate in the biosynthesis of cell wall peptidoglycan, directly affecting the shape, quantity and strength of bacterial cell wall. D-AAs play an important role in bacterial characterization, drug bacteriostasis, and target determination. At present, the mechanism that exogenous addition of D-AAs is involved in peptidoglycan synthesis has been studied and fluorescent derivatives applied to bacterial visualization, specifically detecting cell wall formation/remodeling, bacterial growth and cell morphology. However, the mechanism of how D-AAs affect bacterial growth and its resistance to stress has not been elucidated. The current studies of D-AAs was summarized. The biosynthesis pathway of D-AAs in bacteria, mechanism of cell wall synthesis and the effects of non-canonical D-amino acids on bacterial regulation, and its application in bacterial visualization were highlighted. In the end, the future research direction of D-AAs are prospected.
Key words: Peptidoglycan    D-amino acid    Amino acid isomerase    Amino acid aminotransferase
收稿日期: 2018-11-08 出版日期: 2019-05-08
ZTFLH:  Q517  
基金资助: * 国家自然科学基金资助项目(31570089)
通讯作者: 财音青格乐     E-mail: qinggele@tju.edu.cn
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引用本文:

薛二淑,吴昊,宋倩倩,田开仁,乔建军,财音青格乐. 细菌中D-氨基酸生物合成及调控作用研究进展 *[J]. 中国生物工程杂志, 2019, 39(4): 106-113.

Er-shu XUE,Qian-qian SONG,Kai-ren TIAN,Jian-jun QIAO,Cai-yin QINGGELE. Research Progress in the Biosynthesis and Regulation of D-amino Acids in Bacterial. China Biotechnology, 2019, 39(4): 106-113.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20190414    或    https://manu60.magtech.com.cn/biotech/CN/Y2019/V39/I4/106

图1  D-AAs合成PG侧链[5, 19]
图2  D-AAs的合成
图3  PG的生物合成机制[4-5, 15]
图4  NCDAAs参与PG合成的机制[4-5, 15]
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